Population balance between producing and nonproducing hybridoma clones is very sensitive to serum level, state of inoculum, and medium composition
dc.contributor.author | Chuck, Alice S. | en_US |
dc.contributor.author | Palsson, Bernhard Ø | en_US |
dc.date.accessioned | 2006-04-28T16:30:52Z | |
dc.date.available | 2006-04-28T16:30:52Z | |
dc.date.issued | 1992-02-05 | en_US |
dc.identifier.citation | Chuck, Alice S.; Palsson, Bernhard O. (1992)."Population balance between producing and nonproducing hybridoma clones is very sensitive to serum level, state of inoculum, and medium composition." Biotechnology and Bioengineering 39(3): 354-360. <http://hdl.handle.net/2027.42/37915> | en_US |
dc.identifier.issn | 0006-3592 | en_US |
dc.identifier.issn | 1097-0290 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/37915 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18600953&dopt=citation | en_US |
dc.description.abstract | Secreting and nonsecreting hybridoma populations derived from the murine hybridoma cell line 167.4G5.3 were each grown in batch culture in low serum and serum-free media. Under serum-free conditions, a secreting population gained on a predominantly nonsecreting population and competed with the existing antibody-deficient cells effectively. It was found that this competition was sensitive to state of inoculum and medium composition. We conclude that the competition between a secreting and nonsecreting, or more generally, a producing and nonproducing, population is important; the appearance of the latter may not be a significant setback in terms of expected product titer. | en_US |
dc.format.extent | 609209 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Population balance between producing and nonproducing hybridoma clones is very sensitive to serum level, state of inoculum, and medium composition | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Cellular Biotechnology Laboratory, Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109 | en_US |
dc.contributor.affiliationum | Cellular Biotechnology Laboratory, Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109 ; Cellular Biotechnology Laboratory, Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109 | en_US |
dc.identifier.pmid | 18600953 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/37915/1/260390315_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/bit.260390315 | en_US |
dc.identifier.source | Biotechnology and Bioengineering | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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