Loss of antibody productivity is highly reproducible in multiple hybridoma subclones

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dc.contributor.author Merritt, Steven E. en_US
dc.contributor.author Palsson, Bernhard Ø en_US
dc.date.accessioned 2006-04-28T16:31:23Z
dc.date.available 2006-04-28T16:31:23Z
dc.date.issued 1993-06-20 en_US
dc.identifier.citation Merritt, Steven E.; Palsson, Bernhard O. (1993)."Loss of antibody productivity is highly reproducible in multiple hybridoma subclones." Biotechnology and Bioengineering 42(2): 247-250. <http://hdl.handle.net/2027.42/37925> en_US
dc.identifier.issn 0006-3592 en_US
dc.identifier.issn 1097-0290 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/37925
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18612986&dopt=citation en_US
dc.description.abstract An immunoglobulin G (IgG 2b ) producing hybridoma cell line (S3H5/Γ2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation to serum-free medium, it was found that 16 of the 20 subclones remained at a relatively constant proportion of nonproducing cells. Three of the remaining subclones transiently deviated from this balance but eventually returned toward this population composition. One subclone continued to lose productivity. A population balance was reached at approximately 8% of the population being nonproducers. The loss of antibody productivity was thus highly reproducible. © 1993 John Wiley & Sons, Inc. en_US
dc.format.extent 296712 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language.iso en_US
dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Chemistry en_US
dc.subject.other Biochemistry and Biotechnology en_US
dc.title Loss of antibody productivity is highly reproducible in multiple hybridoma subclones en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Biological Chemistry en_US
dc.subject.hlbsecondlevel Ecology and Evolutionary Biology en_US
dc.subject.hlbsecondlevel Mathematics en_US
dc.subject.hlbsecondlevel Natural Resources and Environment en_US
dc.subject.hlbsecondlevel Statistics and Numeric Data en_US
dc.subject.hlbsecondlevel Public Health en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.subject.hlbtoplevel Social Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Cellular Biotechnology Laboratory, Department of Chemical Engineering, The University of Michigan, Ann Arbor, Michigan 48109 en_US
dc.contributor.affiliationum Cellular Biotechnology Laboratory, Department of Chemical Engineering, The University of Michigan, Ann Arbor, Michigan 48109 ; Cellular Biotechnology Laboratory, Department of Chemical Engineering, The University of Michigan, Ann Arbor, Michigan 48109 en_US
dc.identifier.pmid 18612986 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/37925/1/260420213_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/bit.260420213 en_US
dc.identifier.source Biotechnology and Bioengineering en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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