In vitro synergistic, antagonistic, and duration of exposure effects of metal cations on eukaryotic cells
dc.contributor.author | Wataha, John C. | en_US |
dc.contributor.author | Hanks, Carl T. | en_US |
dc.contributor.author | Craig, Robert G. | en_US |
dc.date.accessioned | 2006-04-28T16:35:11Z | |
dc.date.available | 2006-04-28T16:35:11Z | |
dc.date.issued | 1992-10 | en_US |
dc.identifier.citation | Wataha, J. C.; Hanks, C. T.; Craig, R. G. (1992)." In vitro synergistic, antagonistic, and duration of exposure effects of metal cations on eukaryotic cells." Journal of Biomedical Materials Research 26(10): 1297-1309. <http://hdl.handle.net/2027.42/38000> | en_US |
dc.identifier.issn | 0021-9304 | en_US |
dc.identifier.issn | 1097-4636 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/38000 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1429749&dopt=citation | en_US |
dc.description.abstract | Effects of duration of exposure of single metal cations (Ag +1 , Cd +2 , Cu +2 , Ga +3 , Ni +2 , and Zn +2 ) on 3H-thymidine (3H-Tdr) incorporation and 3-[4,5-dimethylthiazol-2yl-]-2,5-diphenyl tetrazolium bromide-formazan (MTT-f) production in cultured murine fibroblasts (Balb/c 3T3) were investigated, and the synergistic and antagonistic effects of two metal cations applied simultaneously to the fibroblasts were assessed. The effects of duration of exposure were quantified using TC50 values (concentration of an element required to cause 50% toxicity compared with controls) measured after 24, 48, or 72 h. Using MTT-f production, Cd +2 , Cu +2 , Ga +3 , and Ni +2 showed significantly lower TC50 values with increasing time of exposure, whereas the TC50 values for Ag +1 and Zn +2 remained constant. The TC50 values using 3 H-Tdr incorporation exhibited a similar pattern with time of exposure, but the effects were less pronounced. The TC50 values for 3 H-Tdr and MTT-f tended to equalize at 72 h. All combinations of cations tested (Ag-Cu, Ag-Zn, Ag-Ni, Cu-Zn, Cu-Ni, Cu-Ni, Ga-Ni, and Cu-Cd) exhibited synergistic and antagonistic effects were not necessarily mutually exclusive in the same system. © 1992 John Wiley & Sons, Inc. | en_US |
dc.format.extent | 685382 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | In vitro synergistic, antagonistic, and duration of exposure effects of metal cations on eukaryotic cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078 ; The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078 | en_US |
dc.contributor.affiliationum | The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078 | en_US |
dc.contributor.affiliationum | The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078 | en_US |
dc.identifier.pmid | 1429749 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/38000/1/820261004_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jbm.820261004 | en_US |
dc.identifier.source | Journal of Biomedical Materials Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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