Effects of metal ions on osteoblast-like cell metabolism and differentiation
dc.contributor.author | Sun, Zhi Lin | en_US |
dc.contributor.author | Wataha, John C. | en_US |
dc.contributor.author | Hanks, Carl T. | en_US |
dc.date.accessioned | 2006-04-28T16:35:54Z | |
dc.date.available | 2006-04-28T16:35:54Z | |
dc.date.issued | 1997-01 | en_US |
dc.identifier.citation | Sun, Zhi Lin; Wataha, John C.; Hanks, Carl T. (1997)."Effects of metal ions on osteoblast-like cell metabolism and differentiation." Journal of Biomedical Materials Research 34(1): 29-37. <http://hdl.handle.net/2027.42/38014> | en_US |
dc.identifier.issn | 0021-9304 | en_US |
dc.identifier.issn | 1097-4636 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/38014 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8978650&dopt=citation | en_US |
dc.description.abstract | The objective of this study was to evaluate the effects of metal ions, which may be released from orthopedic or dental implants, on osteoblast metabolism and differentiation. ROS 17/2.8 cells were cultured in F-12 medium for 7 days. Then Al +3 , Co +2 , Cr +3 , Ni +2 , Ti +4 , and V +3 were added at concentrations less than their cytotoxic concentrations. After 3 days, DNA synthesis, succinate dehydrogenase activity, alkaline phosphatase (ALP) activity, and culture calcification were assessed. Northern blots were performed for ALP, osteocalcin (OCN), and osteopontin (OPN) mRNA transcription. The data indicated that Cr +3 and Al +3 had few inhibitory effects on ROS cell metabolism below their cytotoxic concentrations, Ni +2 , Co +2 , Ti +4 , and V +3 affected all these parameters of ROS cell metabolism at concentrations below cytotoxic levels. For RNA analysis, Al +3 significantly suppressed the expression of ALP, OCN, and OPN at both cytotoxic and noncytoxic concentrations. Co +2 specifically suppressed ALP expression at cytotoxic concentrations. Cr +3 and Ni +2 inhibited OCN, OPN, and ALP gene expression only at cytotoxic concentrations. For Ti +4 and V +3 ions, gene expression at cytotoxic levels was not significantly affected as compared with the effects at noncytotoxic level. These results show that metal ions may alter osteoblast behavior even at subtoxic concentrations, but do not always affect the expression of all genes similarly. © 1997 John Wiley & Sons, Inc. | en_US |
dc.format.extent | 339086 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Effects of metal ions on osteoblast-like cell metabolism and differentiation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Biological Materials and Sciences and Oral Medicine, Pathology, and Surgery, The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078 ; Department of Biological Materials and Sciences, School of Dentistry Room 5223, University of Michigan, 1011 N. University Avenue, Ann Arbor, MI 48109-1078 | en_US |
dc.contributor.affiliationum | Departments of Biological Materials and Sciences and Oral Medicine, Pathology, and Surgery, The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078 | en_US |
dc.contributor.affiliationother | Department of Oral Rehabilitation, Medical College of Georgia School of Dentistry, Augusta, Georgia 30912-1260 | en_US |
dc.identifier.pmid | 8978650 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/38014/1/5_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-4636(199701)34:1<29::AID-JBM5>3.0.CO;2-P | en_US |
dc.identifier.source | Journal of Biomedical Materials Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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