Ionic effects on strain differences in hepatic cytosolic glucocorticoid receptor levels in mice
dc.contributor.author | Harper, Kenneth J. | en_US |
dc.contributor.author | Erickson, Robert P. | en_US |
dc.date.accessioned | 2006-04-28T16:43:01Z | |
dc.date.available | 2006-04-28T16:43:01Z | |
dc.date.issued | 1983-02 | en_US |
dc.identifier.citation | Harper, Kenneth J.; Erickson, Robert P. (1983)."Ionic effects on strain differences in hepatic cytosolic glucocorticoid receptor levels in mice." Teratology 27(1): 43-49. <http://hdl.handle.net/2027.42/38155> | en_US |
dc.identifier.issn | 0040-3709 | en_US |
dc.identifier.issn | 1096-9926 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/38155 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6845216&dopt=citation | en_US |
dc.description.abstract | Ionic conditions were varied during homogenization of adult livers and during incubation of hepatic cytosol with glucocorticoid, and the effects on the detection of differences between inbred strains of mice for receptor levels were studied. When homogenized directly in 0.01 M Tris or homogenized in distilled water and then buffered to either 0.05 M Tris or 0.01 M potassium phosphate, A/J glucocorticoid receptor levels were greater than those of B10.A. However, when homogenized directly in 0.05 M Tris or buffered to 0.05 M potassium phosphate after homogenization in distilled water, A/J and B10.A had similar receptor levels. When buffered to 0.01 M Tris after homogenization in distilled water, A/J receptor levels were greater than those of B10.A but not significantly so. In Tris buffers, glucocorticoid receptor levels were higher when livers were homogenized directly in buffer than when liver homogenates were buffered after homogenization. This concentration effect is apparently due to the more rapid decay of glucocorticoid receptor in the lower molarity buffer. A reducing agent, thioglycerol, did not appear to affect either the rate of decay of the receptors or the measured level of receptor for B10.A and A/J. C57BL/6J receptor levels were measured in Tris buffers and had a similar relationship to A/J as did B10.A. | en_US |
dc.format.extent | 510903 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Ionic effects on strain differences in hepatic cytosolic glucocorticoid receptor levels in mice | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan School of Medicine, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan School of Medicine, Ann Arbor, Michigan 48109 ; Department of Human Genetics, Box 015, University of Michigan Medical School, 1137 E. Catherine Street, Ann Arbor, MI 48109 | en_US |
dc.identifier.pmid | 6845216 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/38155/1/1420270107_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/tera.1420270107 | en_US |
dc.identifier.source | Teratology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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