Chloroquine embryotoxicity in the postimplantation rat conceptus in vitro
dc.contributor.author | Ambroso, Jeffrey L. | en_US |
dc.contributor.author | Harris, Craig | en_US |
dc.date.accessioned | 2006-04-28T16:43:34Z | |
dc.date.available | 2006-04-28T16:43:34Z | |
dc.date.issued | 1993-09 | en_US |
dc.identifier.citation | Ambroso, Jeffrey L.; Harris, Craig (1993)."Chloroquine embryotoxicity in the postimplantation rat conceptus in vitro." Teratology 48(3): 213-226. <http://hdl.handle.net/2027.42/38166> | en_US |
dc.identifier.issn | 0040-3709 | en_US |
dc.identifier.issn | 1096-9926 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/38166 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8248859&dopt=citation | en_US |
dc.description.abstract | The embryotoxicity of the antimalarial drug chloroquine (CQ) was evaluated in vitro using the rat whole embryo culture system. CQ was found to be embryotoxic and dysmorphogenic when added directly to the culture media containing gestational day (GD) 10 rat conceptuses. Twenty-six-hr exposure to CQ elicited dose-related decreases in embryonic crown-rump length, protein and DNA contents and increases in the incidence of morphologically abnormal embryos. At 30 ΜM CQ, embryonic protein content was decreased to 67% and DNA content to 58% of control while the incidence of morphological abnormalities rose to 100%. Abnormal axial rotation, microophthalmia, and selective cephalic hypoplasia were the most common developmental abnormalities observed. Visceral yolk sac (VYS) vasculature and blood pigmentation were also decreased in a dose-dependent manner, as was VYS DNA content (80% of control at 30 ΜM). VYS protein content, however, showed an alternate pattern of response, decreasing to 87% of control at 10 ΜM CQ but increasing to 125% of control at 30 ΜM. Histologic evaluation revealed that the cytoplasm of the VYS endoderm epithelium was distended due to vacuolization produced by CQ exposure. In the embryo proper, CQ inhibited cranial neural tube development and altered the morphology of cranial neural crest cells. These observations document the in vitro embryotoxicity of CQ and suggest altered VYS histiotrophic nutrition as well as direct embryonic effects as possible mechanisms of CQ embryotoxicity. © 1993 Wiley-Liss, Inc. | en_US |
dc.format.extent | 1230175 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Chloroquine embryotoxicity in the postimplantation rat conceptus in vitro | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Toxicology Program, Department of Environmental and Industrial Health, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Toxicology Program, Department of Environmental and Industrial Health, University of Michigan, Ann Arbor, Michigan 48109 ; Toxicology Program, Department of Environmental and Industrial Health, University of Michigan, 1420 Washington Hts., Ann Arbor, MI 48109-2029 | en_US |
dc.identifier.pmid | 8248859 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/38166/1/1420480305_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/tera.1420480305 | en_US |
dc.identifier.source | Teratology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.