Further insights into sinusoidal organic anion uptake
dc.contributor.author | Gumucio, Jorge J. | en_US |
dc.contributor.author | Moseley, Richard H. | en_US |
dc.date.accessioned | 2006-04-28T16:54:49Z | |
dc.date.available | 2006-04-28T16:54:49Z | |
dc.date.issued | 1992-01 | en_US |
dc.identifier.citation | Gumucio, Jorge J.; Moseley, Richard H. (1992)."Further insights into sinusoidal organic anion uptake." Hepatology 15(1): 163-164. <http://hdl.handle.net/2027.42/38370> | en_US |
dc.identifier.issn | 0270-9139 | en_US |
dc.identifier.issn | 1527-3350 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/38370 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1727792&dopt=citation | en_US |
dc.description.abstract | Previous studies in cultured rat hepatocytes revealed that initial uptake of sulfobromophthalein (BSP) was markedly reduced upon removal of Cl − from the medium. In the present study, unidirectional Cl − gradients were established in short-term cultured rat hepatocytes and their effect on BSP uptake was determined. These investigations revealed that BSP uptake requires external Cl − and is not stimulated by unidirectional Cl − gradients, suggesting that BSP transport is not coupled to Cl − transport. In contrast, BSP transport is stimulated by an inside-to-outside OH − gradient, consistent with OH − exchange or H + cotransport. As the presence of Cl − is essential for but not directly coupled to BSP transport, binding of 35 S-BSP to hepatocytes was determined at 4° C. This revealed an ∼ 10-fold higher affinity of cells for BSP in the presence as compared to the absence of Cl − (K a + 3.2 ± 0.8 vs. 0.42 ± 0.09 ΜM −1 ; P < 0.02). Affinity of BSP for albumin was Cl − -independent, and was ∼ 10% of its affinity for cells in the presence of Cl − . These results indicate that extracellular Cl − modulates the affinity of BSP for its hepatocyte transporter. | en_US |
dc.format.extent | 281534 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | W.B. Saunders | en_US |
dc.publisher | Wiley Periodiocals, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Hepatology | en_US |
dc.title | Further insights into sinusoidal organic anion uptake | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine Division of Gastroenterology (111D) VA Medical Center/University of Michigan Ann Arbor, Michigan 48105 | en_US |
dc.contributor.affiliationum | Veterans Affairs Medical Center and University of Michigan Medical Center, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 1727792 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/38370/1/1840150127_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/hep.1840150127 | en_US |
dc.identifier.source | Hepatology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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