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Role of Na,K-ATPase in regulating acidification of early rat liver endocytic vesicles

dc.contributor.authorAnbari, Maanen_US
dc.contributor.authorRoot, Karen V.en_US
dc.contributor.authorVan Dyke, Rebecca W.en_US
dc.date.accessioned2006-04-28T16:56:36Z
dc.date.available2006-04-28T16:56:36Z
dc.date.issued1994-04en_US
dc.identifier.citationAnbari, Maan; Root, Karen V.; Van Dyke, Rebecca W. (1994)."Role of Na,K-ATPase in regulating acidification of early rat liver endocytic vesicles." Hepatology 19(4): 1034-1043. <http://hdl.handle.net/2027.42/38406>en_US
dc.identifier.issn0270-9139en_US
dc.identifier.issn1527-3350en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/38406
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7511127&dopt=citationen_US
dc.description.abstractEndocytic vesicles are acidified by an electrogenic proton pump and a parallel chloride conductance; however, acidification might be decreased if electrogenic transporters, such as Na,K-ATPase, that increase vesicle interior-positive membrane potential were also present. We examined this issue in early rat liver endosomes using ion substitution and inhibitors to alter Na,K-ATPase activity. These early endosomes, labeled for 2 min with the fluorescent fluid-phase marker fluorescein isothiocyanate-dextran, consistently acidified faster than endosomes similarily labeled for a 10-min period. In chloride-free media initial rates of acidification of early endosomes were faster in K + media than in Na + medium, although addition of K + to Na + or Na + to K + media to allow Na,K-ATPase to function did not decrease the rate of acidification. In chloride-containing media, rates were the same regardless of cation composition. The Na,K-ATPase inhibitor vanadate was prepared from orthovanadate by several methods, all of which inhibited liver ATPase activity. Two hundred Μmol/L vanadate, prepared Cl − -free, tended to decrease rates of acidification in all media tested and these effects achieved statistical significance in Cl − -free media containing 150 mmol/L K + or mixtures of Na + and K + and in 145 mmol/L KCl/5 mmol/L NaCl medium. Vanadate stocks pH-adjusted with hydrogen chloride increased rates of acidification in sodium gluconate buffers, probably as a result of the effects of the included Cl − . Five mmol/L ouabain (loaded into vesicles by endocytosis) and the membrane-permeable analog strophanthidin (2 mmol/L) both markedly inhibited endosome acidification, regardless of buffer ion composition. Collectively, these results suggest that Na,K-ATPase does not regulate acidification of rat liver early endocytic vesicles, that vanadate may modestly inhibit endosome acidification and that ouabain at high concentrations may inhibit acidification from the vesicle interior face. (H EPATOLOGY 1994;19:1034–1043.)en_US
dc.format.extent1328796 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherW.B. Saundersen_US
dc.publisherWiley Periodiocals, Inc.en_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherHepatologyen_US
dc.titleRole of Na,K-ATPase in regulating acidification of early rat liver endocytic vesiclesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelInternal Medicine and Specialtiesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDivision of Gastroenterology, Department of Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan 48109-0682en_US
dc.contributor.affiliationumDivision of Gastroenterology, Department of Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan 48109-0682en_US
dc.contributor.affiliationumDivision of Gastroenterology, Department of Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan 48109-0682 ; 6520 MSRB-I, University of Michigan Medical Center, 1150 W. Medical Center Dr., Ann Arbor, MI 48109-0682en_US
dc.identifier.pmid7511127en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/38406/1/1840190432_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/hep.1840190432en_US
dc.identifier.sourceHepatologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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