Evidence for a regulatory element controlling amino aced transport system L in Chinese hamster ovary cells
dc.contributor.author | Collarini, Ellen J. | en_US |
dc.contributor.author | Campbell, George S. | en_US |
dc.contributor.author | Oxender, Dale L. | en_US |
dc.date.accessioned | 2006-04-28T16:59:10Z | |
dc.date.available | 2006-04-28T16:59:10Z | |
dc.date.issued | 1994-12 | en_US |
dc.identifier.citation | Collarini, Ellen J.; Campbell, George S.; Oxender, Dale L. (1994)."Evidence for a regulatory element controlling amino aced transport system L in Chinese hamster ovary cells." Journal of Cellular Biochemistry 56(4): 544-549. <http://hdl.handle.net/2027.42/38457> | en_US |
dc.identifier.issn | 0730-2312 | en_US |
dc.identifier.issn | 1097-4644 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/38457 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7890812&dopt=citation | en_US |
dc.description.abstract | We have used the technique of somatic cell hybridization to study the regulation of the neutral amino acid transport system L in Chinese hamster ovary (CHO) cells. The cell line CHO–; ts O25C1 has a temperature-sinsitive mutationin leucyl-tRNA synthetase. At the nonpermissive temperature of 39 o C, CHO– ts O25C1 cells are unable to charge leucyl-tRNA and behave as though starved for leucine by increasing their system L transport activity two- to fourfold. From the temperature-sensitive cell line, we have isolated a regulatory mutant cell, CHO–C11B6, that has constitutively elevated system L transport activity. The CHO–C11B6 cell line retains the temperature-sensitive leucyl-tRNA synthetase mutation, but growth of this cell line is temperature resistant because its increased system L transport activity leads of increased intracellular leucine levels, which compensate for the defective. Hybrid cells formed by fusion of the temperature-sensitive CHO–; ts O25C1 cells the temperature-resistant CHO–C11B6 cells show temperature-sensitive growth and temperature-dependent regulation of leucine transport activity. These data suggest that the system L activity of CHO cells is regulated by a dominant-acting element that is defective or absent in the regulatory mutant CHO–C11B6 cell line. | en_US |
dc.format.extent | 599679 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Evidence for a regulatory element controlling amino aced transport system L in Chinese hamster ovary cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 ; Laboratory for Molecular Cell Biology, Gower Street, University College, London WClE 6BT, England | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 7890812 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/38457/1/240560415_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jcb.240560415 | en_US |
dc.identifier.source | Journal of Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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