Preferential suppression of insulin-stimulated proliferation of cultured hepatocytes by somatostatin: Evidence for receptor-mediated growth regulation
dc.contributor.author | Kothary, Piyush C. | en_US |
dc.contributor.author | Kokudo, Norihiro | en_US |
dc.contributor.author | Eckhauser, Frederic E. | en_US |
dc.contributor.author | DelValle, John | en_US |
dc.contributor.author | Raper, Steven E. | en_US |
dc.date.accessioned | 2006-04-28T16:59:13Z | |
dc.date.available | 2006-04-28T16:59:13Z | |
dc.date.issued | 1995-10 | en_US |
dc.identifier.citation | Kothary, Piyush C.; Kokudo, Norihiro; Eckhauser, F. E.; Delvalle, John; Raper, Steven E. (1995)."Preferential suppression of insulin-stimulated proliferation of cultured hepatocytes by somatostatin: Evidence for receptor-mediated growth regulation." Journal of Cellular Biochemistry 59(2): 258-265. <http://hdl.handle.net/2027.42/38458> | en_US |
dc.identifier.issn | 0730-2312 | en_US |
dc.identifier.issn | 1097-4644 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/38458 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8904319&dopt=citation | en_US |
dc.description.abstract | The role of somatostatin (SS-14) in the regulation of rat liver regeneration was examined by using thymidine incorporation into hepatocyte DNA labeled with tritiated thymidine, a nuclear-labeling index, and the binding of 125 I-tyr 11 -SS-14 to hepatocytes isolated at various times after partial hepatectomy. The data demonstrated no suppressive effect of SS-14 on insulin and glucagon-stimulated thymidine incorporation into hepatocyte DNA as early as 2 h after partial hepatectomy. These data were substantiated by a nuclear labeling index studies. At 2 h, 125 I-tyr 11 -SS-14 binding to its specific sites on isolated hepatocytes was undetectable. There was a time-dependent increase in binding of 125 I-tyr 11 -SS-14 to hepatocytes obtained at various times after partial hepatectomy. There was a significant decrease in the number of binding sites after partial hepatectomy as determined by Scatchard analysis. The data were supported by autoradiography analysis of affinity labeled 125 I-tyr 11 -SS-14-binding protein complex followed by SDS-PAGE. SS-14 also inhibited intracellular cAMP in hepatocytes obtained at 18 h after hepatectomy. The data are consistent with the hypothesis that SS-14 participates via its own receptor in the regulation of the liver regeneration. © 1995 Wiley-Liss, Inc. | en_US |
dc.format.extent | 795770 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Preferential suppression of insulin-stimulated proliferation of cultured hepatocytes by somatostatin: Evidence for receptor-mediated growth regulation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Ophthalmology, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0714 ; 517 Kellogg Eye Center, University of Michigan Medical Center, Ann Arbor, MI 48109-0714 | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0714 | en_US |
dc.contributor.affiliationum | Department of Medicine, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0714 | en_US |
dc.contributor.affiliationother | Department of Surgery, University of Tokyo, Tokyo, Japan | en_US |
dc.contributor.affiliationother | Howard Hughes Institute, University of Pennsylvania, Philadelphia, Pennsylvania | en_US |
dc.identifier.pmid | 8904319 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/38458/1/240590214_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jcb.240590214 | en_US |
dc.identifier.source | Journal of Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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