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Detection of human somatic cell structural gene mutations by two-dimensional electrophoresis
dc.contributor.authorHanash, Samir M.en_US
dc.contributor.authorChu, Ernest H. Y.en_US
dc.contributor.authorKuick, Rorken_US
dc.contributor.authorSkolnick, M.en_US
dc.contributor.authorNeel, James V.en_US
dc.contributor.authorStrahler, John R.en_US
dc.contributor.authorPivirotto, S.en_US
dc.contributor.authorNiezgoda, W.en_US
dc.date.accessioned2006-04-28T17:02:07Z
dc.date.available2006-04-28T17:02:07Z
dc.date.issued1987en_US
dc.identifier.citationHanash, S.M.; Chu, E.H.Y.; Kuick, R.; Skolnick, M.; Neel, J.; Strahler, J.; Pivirotto, S.; Niezgoda, W. (1987)."Detection of human somatic cell structural gene mutations by two-dimensional electrophoresis." Proteins: Structure, Function, and Genetics 2(1): 13-19. <http://hdl.handle.net/2027.42/38517>en_US
dc.identifier.issn0887-3585en_US
dc.identifier.issn1097-0134en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/38517
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3328857&dopt=citationen_US
dc.description.abstractThe feasibility of detecting human somatic structural gene mutations by two dimensional electrophoresis has been investigated. A lymphoblastoid cell line was grown as a mass culture in the presence of ethylnitrosourea, after which cells were regrown as single cell clones. A total of 257 polypeptide spots were analyzed in gels derived from 186 clones. Four structural mutations were detected by visual analysis of the gels. Computer analysis of gels corresponding to the mutant clones was also undertaken. At a spot size threshold of 200 spots to be matched using a computer algorithm, all four mutant polypeptides were detected. These results indicate the usefulness of the two-dimensional approach for mutagenesis studies at the protein level.en_US
dc.format.extent1194392 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherChemistryen_US
dc.subject.otherBiochemistry and Biotechnologyen_US
dc.titleDetection of human somatic cell structural gene mutations by two-dimensional electrophoresisen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pediatrics, The University of Michigan, Ann Arbor, Michigan, 48109 ; Department of Pediatrics, 4451 Kresge 1, University of Michigan, Ann Arbor, MI 48109-0510en_US
dc.contributor.affiliationumDepartment of Human Genetics, The University of Michigan, Ann Arbor, Michigan, 48109en_US
dc.contributor.affiliationumDepartment of Human Genetics, The University of Michigan, Ann Arbor, Michigan, 48109en_US
dc.contributor.affiliationumDepartment of Human Genetics, The University of Michigan, Ann Arbor, Michigan, 48109en_US
dc.contributor.affiliationumDepartment of Pediatrics, The University of Michigan, Ann Arbor, Michigan, 48109en_US
dc.contributor.affiliationumDepartment of Human Genetics, The University of Michigan, Ann Arbor, Michigan, 48109en_US
dc.contributor.affiliationumDepartment of Human Genetics, The University of Michigan, Ann Arbor, Michigan, 48109en_US
dc.contributor.affiliationotherDepartment of Computer Science, Rensselaer Polytechnic Institute, Troy, New York 12180en_US
dc.identifier.pmid3328857en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/38517/1/340020103_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/prot.340020103en_US
dc.identifier.sourceProteins: Structure, Function, and Geneticsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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