Detection of human somatic cell structural gene mutations by two-dimensional electrophoresis
dc.contributor.author | Hanash, Samir M. | en_US |
dc.contributor.author | Chu, Ernest H. Y. | en_US |
dc.contributor.author | Kuick, Rork | en_US |
dc.contributor.author | Skolnick, M. | en_US |
dc.contributor.author | Neel, James V. | en_US |
dc.contributor.author | Strahler, John R. | en_US |
dc.contributor.author | Pivirotto, S. | en_US |
dc.contributor.author | Niezgoda, W. | en_US |
dc.date.accessioned | 2006-04-28T17:02:07Z | |
dc.date.available | 2006-04-28T17:02:07Z | |
dc.date.issued | 1987 | en_US |
dc.identifier.citation | Hanash, S.M.; Chu, E.H.Y.; Kuick, R.; Skolnick, M.; Neel, J.; Strahler, J.; Pivirotto, S.; Niezgoda, W. (1987)."Detection of human somatic cell structural gene mutations by two-dimensional electrophoresis." Proteins: Structure, Function, and Genetics 2(1): 13-19. <http://hdl.handle.net/2027.42/38517> | en_US |
dc.identifier.issn | 0887-3585 | en_US |
dc.identifier.issn | 1097-0134 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/38517 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3328857&dopt=citation | en_US |
dc.description.abstract | The feasibility of detecting human somatic structural gene mutations by two dimensional electrophoresis has been investigated. A lymphoblastoid cell line was grown as a mass culture in the presence of ethylnitrosourea, after which cells were regrown as single cell clones. A total of 257 polypeptide spots were analyzed in gels derived from 186 clones. Four structural mutations were detected by visual analysis of the gels. Computer analysis of gels corresponding to the mutant clones was also undertaken. At a spot size threshold of 200 spots to be matched using a computer algorithm, all four mutant polypeptides were detected. These results indicate the usefulness of the two-dimensional approach for mutagenesis studies at the protein level. | en_US |
dc.format.extent | 1194392 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Detection of human somatic cell structural gene mutations by two-dimensional electrophoresis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics, The University of Michigan, Ann Arbor, Michigan, 48109 ; Department of Pediatrics, 4451 Kresge 1, University of Michigan, Ann Arbor, MI 48109-0510 | en_US |
dc.contributor.affiliationum | Department of Human Genetics, The University of Michigan, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Human Genetics, The University of Michigan, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Human Genetics, The University of Michigan, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Pediatrics, The University of Michigan, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Human Genetics, The University of Michigan, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Human Genetics, The University of Michigan, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationother | Department of Computer Science, Rensselaer Polytechnic Institute, Troy, New York 12180 | en_US |
dc.identifier.pmid | 3328857 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/38517/1/340020103_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/prot.340020103 | en_US |
dc.identifier.source | Proteins: Structure, Function, and Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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