In vivo 31 P MRS evaluation of ganciclovir toxicity in C6 gliomas stably expressing the herpes simplex thymidine kinase gene

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dc.contributor.author Stegman, Lauren D. en_US
dc.contributor.author Ben-Yoseph, Oded en_US
dc.contributor.author Freyer, James P. en_US
dc.contributor.author Ross, Brian D. en_US
dc.date.accessioned 2006-04-28T17:02:57Z
dc.date.available 2006-04-28T17:02:57Z
dc.date.issued 1996-12 en_US
dc.identifier.citation Stegman, Lauren D.; Ben-Yoseph, Oded; Freyer, James P.; Ross, Brian D. (1996)." In vivo 31 P MRS evaluation of ganciclovir toxicity in C6 gliomas stably expressing the herpes simplex thymidine kinase gene." NMR in Biomedicine 9(8): 364-368. <http://hdl.handle.net/2027.42/38534> en_US
dc.identifier.issn 0952-3480 en_US
dc.identifier.issn 1099-1492 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/38534
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9176891&dopt=citation en_US
dc.description.abstract Phosphorus MRS was evaluated as a monitor of tumour therapeutic response to the herpes simplex virus thymidine kinase suicide gene therapy paradigm. In vivo 31 P spectra were obtained from subcutaneous rat C6 gliomas constitutively expressing the HSVtk gene post treatment with gancielovir (GCV, 15 mg/kg i.p., twice-daily). Significant regression ( p <0.1) of tumour volume was observed 10 days after beginning GCV administration. However, no changes in tumour pH or energy metabolites from pre-treatment values were observed. High-resolution 31 P spectra of tumour extracts revealed a statistically significant reduction in the phosphocholine to phosphoethanolamine ratio six days post-GCV administration. These results indicate that the HSVtk/GCV-induced killing of tumours is not associated with corresponding changes in 31 P MRS-observable energy metabolites and pH. The observed reduction in the PE/PC ratio may provide a non-invasive in vivo indicator of therapeutic efficacy. © 1996 by John Wiley & Sons, Ltd. en_US
dc.format.extent 1024 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/octet-stream
dc.format.mimetype text/plain
dc.language.iso en_US
dc.publisher John Wiley & Sons, Ltd. en_US
dc.subject.other Chemistry en_US
dc.subject.other Analytical Chemistry and Spectroscopy en_US
dc.title In vivo 31 P MRS evaluation of ganciclovir toxicity in C6 gliomas stably expressing the herpes simplex thymidine kinase gene en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Electrical Engineering en_US
dc.subject.hlbsecondlevel Physics en_US
dc.subject.hlbtoplevel Engineering en_US
dc.subject.hlbtoplevel Science en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA en_US
dc.contributor.affiliationum Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109, USA en_US
dc.contributor.affiliationum Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA ; Department of Radiology, University of Michigan Medical School, Ann Arbor, MI 48109, USA ; B. D. Ross at Department of Radiology, University of Michigan Medical School, Ann Arbor, USA en_US
dc.contributor.affiliationother Life Sciences Division, Los Alamos National Laboratory, Los Alamos, NM, USA en_US
dc.identifier.pmid 9176891 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/38534/1/sgml.22285 en_US
dc.identifier.doi http://dx.doi.org/10.1002/(SICI)1099-1492(199612)9:8<364::AID-NBM436>3.0.CO;2-W en_US
dc.identifier.source NMR in Biomedicine en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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