Combinatorial gene therapy for bone regeneration: Cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7
dc.contributor.author | Zhao, Ming | en_US |
dc.contributor.author | Zhao, Zhuoran | en_US |
dc.contributor.author | Koh, Jeong-Tae | en_US |
dc.contributor.author | Jin, Taocong | en_US |
dc.contributor.author | Franceschi, Renny T. | en_US |
dc.date.accessioned | 2006-05-17T14:42:50Z | |
dc.date.available | 2006-05-17T14:42:50Z | |
dc.date.issued | 2005-05-01 | en_US |
dc.identifier.citation | Zhao, Ming; Zhao, Zhuoran; Koh, Jeong-Tae; Jin, Taocong; Franceschi, Renny T. (2005)."Combinatorial gene therapy for bone regeneration: Cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7." Journal of Cellular Biochemistry 95(1): 1-16. <http://hdl.handle.net/2027.42/39124> | en_US |
dc.identifier.issn | 0730-2312 | en_US |
dc.identifier.issn | 1097-4644 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/39124 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15759283&dopt=citation | en_US |
dc.description.abstract | Bone morphogenetic proteins (BMPs) have demonstrated effectiveness as bone regeneration agents whether delivered as recombinant proteins or via gene therapy. Current gene therapy approaches use vectors expressing single BMPs. In contrast, multiple BMPs are coordinately expressed during bone development and fracture healing. Furthermore, BMPs likely exist in vivo as heterodimeric molecules having enhanced biological activity. In the present study, we test the hypothesis that gene therapy-based bone regeneration can be enhanced by expressing combinations of BMPs. For in vitro studies, mesenchymal cell lines were transduced with individual adenoviruses containing BMP2, 4, or 7 cDNA under control of a CMV promoter (AdBMP2, 4, 7) or virus combinations. Significantly, combined transduction with AdBMP2 plus AdBMP7 or AdBMP4 plus AdBMP7 resulted in a synergistic stimulation of osteoblast differentiation. This synergy is best explained by formation of BMP2/7 and 4/7 heterodimers. To test in vivo biological activity, fibroblasts were transduced with specific virus combinations and implanted into C57BL6 mice. Consistent with in vitro results, strong synergy was observed using combined AdBMP2/BMP7 treatment, which induced twofold to threefold more bone than would be predicted based on the activity of individual AdBMPs. These studies show that dramatic enhancement of osteogenesis can be achieved using gene therapy to express specific combinations of interacting regenerative molecules. © 2005 Wiley-Liss, Inc. | en_US |
dc.format.extent | 359208 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Combinatorial gene therapy for bone regeneration: Cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Periodontics, Prevention, and Geriatrics, School of Dentistry and Center for Craniofacial Regeneration, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Periodontics, Prevention, and Geriatrics, School of Dentistry and Center for Craniofacial Regeneration, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Molecular Biology Core Laboratory, School of Dentistry, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Periodontics, Prevention, and Geriatrics, School of Dentistry and Center for Craniofacial Regeneration, University of Michigan, Ann Arbor, Michigan ; Department of Biological Chemistry, School of Medicine, University of Michigan, Ann Arbor, Michigan ; Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, 1011 North University Avenue, Ann Arbor, MI 48109-1078. | en_US |
dc.contributor.affiliationother | Chonnam National University, Kwangju, South Korea | en_US |
dc.identifier.pmid | 15759283 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/39124/1/20411_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jcb.20411 | en_US |
dc.identifier.source | Journal of Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.