Now showing items 11-20 of 47
Low-Flow Push-Pull Perfusion for Measuring Neurotransmitters with High Spatial and Temporal Resolution within the Living Brain.
(2013)
Low-flow push-pull perfusion is a technique for measuring neurotransmitters within the brain with ~200 μm resolution. Activity of neurotransmitters can vary on this size scale; therefore, low-flow push-pull may offer new ...
Computational Approaches Enabling Disparately Acquired Untargeted LC-MS Metabolomics Data Analysis
(2022)
Metabolomics is the systematic study of small molecule metabolites that are substrates, intermediates, and products of cellular metabolism. Metabolomics assays performed using liquid chromatography- mass spectrometry (LC-MS) ...
(1) The Relationship of Protein Expression and Cell Division, (2) 3D Imaging of Cells Using Digital Holography, and (3) General Chemistry Enrollment at University of Michigan.
(2012)
Chapter 1: We evaluated the relationship between cell division and protein expression when using commercial poly(ethylenimine) (PEI)-based polyplexes. The membrane dye PKH26 was used to assess cell division, and cyan ...
Functional Analysis of Protein S-Palmitoylation Enzymes.
(2015)
Protein S-palmitoylation is a dynamic, hydrophobic, post-translational modification of cysteine residues that is required for the spatiotemporal organization of hundreds of proteins. In turn, protein palmitoylation contributes ...
Biophysical Approaches to Target Protein-Protein Interactions of Epigenetic Complexes by Small-Molecule Inhibitors
(2019)
Innumerable biological systems rely on Protein-Protein Interactions (PPIs) to govern basal physiological processes. Deregulation of PPIs is implicated in various human diseases, and PPIs have thus been frequently promoted ...
Development of Novel Small Molecule Inhibitors of Neurotropic Alphavirus Replication.
(2014)
Emerging mosquito-borne viruses (arboviruses) constitute a large public health risk because of the ubiquity of mosquitoes and the potential for mosquito-human transmission. Neurotropic alphaviruses are of particular interest ...
Nanopores with Fluid Walls for Characterizing Proteins and Peptides.
(2012)
Nanopore-based, resistive-pulse sensing is a simple single-molecule technique, is label free, and employs basic electronic recording equipment. This technique shows promise for rapid, multi-parameter characterization of ...
Computational Methods for Characterizing Post-translational and Chemical Modifications Found in Open Searches
(2022)
Post-translational modifications (PTMs) govern many processes within cells and understanding their function is critical to both the basic and biomedical sciences. However, identifying modified peptides, particularly ...
Design, Synthesis, and Evaluation of Novel, Multifunctional Dimethyltyrosine-Tetrahydroisoquinoline Opioid Peptidomimetics
(2019)
The opioid receptors modulate a wide variety of physiological and behavioral functions, including pain, mood, and reward. There are three main types of opioid receptors – kappa (KOR), mu (MOR), and delta (DOR), and many ...
Structural and Biochemical Insights into Methylation Site and State Specificity of JMJD2 Lysine Demethylases.
(2012)
The human JMJD2/KDM4 family of histone lysine demethylases comprises four homologs: JMJD2A, JMJD2B, JMJD2C and JMJD2D. These enzymes have been implicated in a number of biological processes such as transcriptional activation, ...