Hydrolysis of γ -glutamyl linkages by Fusobacterium nucleatum
dc.contributor.author | Mäkinen, Kauko K. | en_US |
dc.contributor.author | Söderling, Eva | en_US |
dc.contributor.author | Mäkinen, Pirkko-Liisa | en_US |
dc.contributor.author | Syed, Salam A. | en_US |
dc.date.accessioned | 2006-09-08T19:08:42Z | |
dc.date.available | 2006-09-08T19:08:42Z | |
dc.date.issued | 1990-01 | en_US |
dc.identifier.citation | Mäkinen, Kauko K.; Söderling, Eva; Syed, Salam A.; Mäkinen, Pirkko-Liisa; (1990). "Hydrolysis of γ -glutamyl linkages by Fusobacterium nucleatum ." Current Microbiology 20(1): 5-11. <http://hdl.handle.net/2027.42/41334> | en_US |
dc.identifier.issn | 0343-8651 | en_US |
dc.identifier.issn | 1432-0991 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41334 | |
dc.description.abstract | The cell extracts of two human oral strains (FN2 and FN3) of Fusobacterium nucleatum displayed exceptionally high γ -glutamylpeptidase activity as determined with N-γ - l -glutamyl-2-naphthylamine as substrate. This activity was so dominant that the hydrolysis of other N -aminoacyl-2-naphthylamines progressed at a rate <10% of the former. Two major enzymes (I and II) were partially purified from FN2. I had a molecular weight of 115,000 and did not hydrolyze γ -glutamylcysteinylglycine (glutathione). II had a molecular weight of 70,000 and rapidly liberated only glutamic acid from glutathione. Strain FN3 contained several enzymes hydrolyzing γ -glu-2NA. Direct anion exchange chromatography of FN3 cell extracts separated one enzyme that liberated both glutamic acid and glycine from glutathione, one that was inactive against glutathione (but hydrolyzed γ -glu-2NA), and one that liberated only glutamic acid. Although γ -glu-2NA was a good synthetic substrate, glutathione was hydrolyzed at least 500 times faster by an enzyme present in both strains. These results indicate that the presence of γ -glutamylpeptidase activity is very characteristic of these F. nucleatum strains. | en_US |
dc.format.extent | 693961 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag New York Inc. | en_US |
dc.subject.other | Biotechnology | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Microbiology | en_US |
dc.title | Hydrolysis of γ -glutamyl linkages by Fusobacterium nucleatum | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | School of Dentistry, Department of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | School of Dentistry, Department of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | School of Dentistry, Department of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | School of Dentistry, Department of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41334/1/284_2005_Article_BF02094016.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF02094016 | en_US |
dc.identifier.source | Current Microbiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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