Genetic regulation of glycogen biosynthesis in Escherichia coli : In vivo effects of the catabolite repression and stringent response systems in glg gene expression
dc.contributor.author | Romeo, Tony | en_US |
dc.contributor.author | Black, Jill | en_US |
dc.contributor.author | Preiss, Jack | en_US |
dc.date.accessioned | 2006-09-08T19:08:53Z | |
dc.date.available | 2006-09-08T19:08:53Z | |
dc.date.issued | 1990-08 | en_US |
dc.identifier.citation | Romeo, Tony; Black, Jill; Preiss, Jack; (1990). "Genetic regulation of glycogen biosynthesis in Escherichia coli : In vivo effects of the catabolite repression and stringent response systems in glg gene expression." Current Microbiology 21(2): 131-137. <http://hdl.handle.net/2027.42/41337> | en_US |
dc.identifier.issn | 1432-0991 | en_US |
dc.identifier.issn | 0343-8651 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41337 | |
dc.description.abstract | The synthesis of two of the Escherichia coli glycogen biosynthetic enzymes, ADPglucose pyrophosphorylase ( glg C) and glycogen synthase ( glg A) was activated by the addition of 5 m M cyclic AMP (cAMP) to maxicells; synthesis of glycogen branching enzyme ( glg B) was unaffected. β -Galactosidase activity expressed from a gene fusion, φ(glg C- lac Z), was approximately five-fold higher in a cya + versus an isogenic cya − strain of E. coli . Addition of cAMP restored β -galactosidase in the cya − strain. The expression of φ(glg C‘−’ lac Z) encoded β -galactosidase activity in a series of spo T mutants exhibited an apparent exponential relationship to intracellular guanosine 5′-diphosphate 3′-diphosphate (ppGpp) levels. These results provide evidence for the control of glycogen biosynthesis in vivo by cAMP and ppGpp at the level of gene expression, and identify a region of DNA required for the control. The φ(glg C‘−’ lac Z) encoded β -galactosidase activity was also elevated three-to five-fold in strain AC70R1, which contains a transacting mutation ( glg Q) that affects the levels of the glycogen biosynthetic enzymes and glg C transcripts. | en_US |
dc.format.extent | 727334 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag New York Inc. | en_US |
dc.subject.other | Biotechnology | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Microbiology | en_US |
dc.title | Genetic regulation of glycogen biosynthesis in Escherichia coli : In vivo effects of the catabolite repression and stringent response systems in glg gene expression | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biochemistry, Michigan State University, 48824, East Lansing, MI, USA; Department of Pharmacology, University of Michigan, 48104, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Biochemistry, Michigan State University, 48824, East Lansing, MI, USA | en_US |
dc.contributor.affiliationother | Department of Biochemistry, Michigan State University, 48824, East Lansing, MI, USA; Department of Microbiology and Immunology, Texas College of Osteopathic Medicine, 76107-2690, Fort Worth, TX, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41337/1/284_2005_Article_BF02091831.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF02091831 | en_US |
dc.identifier.source | Current Microbiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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