Immortalized Mouse Inner Ear Cell Lines Demonstrate a Role for Chemokines in Promoting the Growth of Developing Statoacoustic Ganglion Neurons
dc.contributor.author | Daruwalla, Zeeba | en_US |
dc.contributor.author | Roth, Therese Mary | en_US |
dc.contributor.author | Bianchi, Lynne M. | en_US |
dc.contributor.author | Allen, Susan J. | en_US |
dc.contributor.author | White, Ian O. | en_US |
dc.contributor.author | Lukacs, Nicholas W. | en_US |
dc.contributor.author | Barald, Kate F. | en_US |
dc.contributor.author | Richards, Ayo-Lynn | en_US |
dc.contributor.author | Attia, Naweah P. | en_US |
dc.date.accessioned | 2006-09-08T19:12:13Z | |
dc.date.available | 2006-09-08T19:12:13Z | |
dc.date.issued | 2005-12 | en_US |
dc.identifier.citation | Bianchi, Lynne M.; Daruwalla, Zeeba; Roth, Therese M.; Attia, Naweah P.; Lukacs, Nicholas W.; Richards, Ayo-Lynn; White, Ian O.; Allen, Susan J.; Barald, Kate F.; (2005). "Immortalized Mouse Inner Ear Cell Lines Demonstrate a Role for Chemokines in Promoting the Growth of Developing Statoacoustic Ganglion Neurons." Journal of the Association for Research in Otolaryngology 6(4): 355-367. <http://hdl.handle.net/2027.42/41388> | en_US |
dc.identifier.issn | 1438-7573 | en_US |
dc.identifier.issn | 1525-3961 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41388 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16240240&dopt=citation | en_US |
dc.description.abstract | The target-derived factors necessary for promoting initial outgrowth from the statoacoustic ganglion (SAG) to the inner ear have not been fully characterized. In the present study, conditioned medium from embryonic Immortomouse inner ear cell lines that maintain many characteristics of developing inner ear sensory epithelia were screened for neurite-promoting activity. Conditioned medium found to be positive for promoting SAG neurite outgrowth and neuronal survival was then tested for the presence of chemokines, molecules that have not previously been investigated for promoting SAG outgrowth. One candidate molecule, monocyte chemotactic protein 1 (MCP-1), was detected in the conditioned medium and subsequently localized to mouse hair cells by immunocytochemistry. In vitro studies demonstrated that function-blocking MCP-1 antibodies decreased the amount of SAG neurite outgrowth induced by the conditioned medium and that subsequent addition of MCP-1 protein was able to promote outgrowth when added to the antibody-treated conditioned medium. The use of the Immortomouse cell lines proved valuable in identifying this candidate cofactor that promotes outgrowth of early-stage SAG nerve fibers and is expressed in embryonic hair cells. | en_US |
dc.format.extent | 382385 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Association for Research in Otolaryngology | en_US |
dc.subject.other | Immortomouse | en_US |
dc.subject.other | Cochlea | en_US |
dc.subject.other | Auditory | en_US |
dc.subject.other | Neural | en_US |
dc.subject.other | Spiral Ganglion | en_US |
dc.subject.other | Development | en_US |
dc.title | Immortalized Mouse Inner Ear Cell Lines Demonstrate a Role for Chemokines in Promoting the Growth of Developing Statoacoustic Ganglion Neurons | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Otolaryngology | en_US |
dc.subject.hlbsecondlevel | Otolaryngology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA, | en_US |
dc.contributor.affiliationum | Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA, | en_US |
dc.contributor.affiliationum | Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA, ; Program in Neuroscience, University of Michigan Medical School, Ann Arbor, MI, USA, ; Cell and Molecular Biology, University of Michigan Medical School, Ann Arbor, MI, USA, | en_US |
dc.contributor.affiliationum | Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA, | en_US |
dc.contributor.affiliationum | Neuroscience Department, Oberlin College, Oberlin, OH, 44074, USA, ; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA, | en_US |
dc.contributor.affiliationum | Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA, | en_US |
dc.contributor.affiliationum | Neuroscience Department, Oberlin College, Oberlin, OH, 44074, USA, ; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA, | en_US |
dc.contributor.affiliationother | Neuroscience Department, Oberlin College, Oberlin, OH, 44074, USA, | en_US |
dc.contributor.affiliationother | Neuroscience Department, Oberlin College, Oberlin, OH, 44074, USA, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 16240240 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41388/1/10162_2005_Article_13.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s10162-005-0013-8 | en_US |
dc.identifier.source | Journal of the Association for Research in Otolaryngology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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