Synthesis and Receptor Binding Studies of ( ± )l-Iodo-MK-801
dc.contributor.author | Yang, David J. | en_US |
dc.contributor.author | Wieland, Donald M. | en_US |
dc.contributor.author | Ciliax, Brian J. | en_US |
dc.contributor.author | Gildersleeve, David L. | en_US |
dc.contributor.author | Van Dort, Marcian E. | en_US |
dc.contributor.author | Young, Anne B. | en_US |
dc.contributor.author | Pirat, Jean-Luc | en_US |
dc.date.accessioned | 2006-09-08T19:22:00Z | |
dc.date.available | 2006-09-08T19:22:00Z | |
dc.date.issued | 1989-06 | en_US |
dc.identifier.citation | Yang, David J.; Ciliax, Brian J.; Van Dort, Marcian E.; Gildersleeve, David L.; Pirat, Jean-Luc; Young, Anne B.; Wieland, Donald M.; (1989). "Synthesis and Receptor Binding Studies of ( ± )l-Iodo-MK-801." Pharmaceutical Research 6(6): 474-476. <http://hdl.handle.net/2027.42/41537> | en_US |
dc.identifier.issn | 1573-904X | en_US |
dc.identifier.issn | 0724-8741 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41537 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2548173&dopt=citation | en_US |
dc.description.abstract | The glutamate analogue N -methyl-D-aspartate (NMDA) binds to a subset of glutamate receptors that are coupled to a voltage-sensitive cation channel. This NMDA-linked channel is the likely binding locus of the potent anticonvulsant MK-801. To develop single-photon emission computed tomography (SPECT) probes of this brain channel, we synthesized (±)l-iodo-MK-801 and (±)l-[ 125 I]iodo-MK-801. The effect of (±)l-iodo-MK-801 on ligand binding to the NMDA-linked glutamate receptor site was assessed using a rat brain homogenate assay. (±)l-Iodo-MK-801 displaced the dissociative anesthetic ligand [ 3 H] N -[l-(2-thienyl)cyclohexyl]piperidine ([ 3 H]TCP) binding with an IC 50 of 1 µ M , which is a 10-fold lower binding affinity than that of (±)MK-801. In in vivo autoradiographic studies, (±)MK-801 failed to block selective uptake of (±)l-iodo-MK-801 in rat brain. These results suggest that (±)l-iodo-MK-801 may not be a suitable ligand for mapping NMDA-linked glutamate receptor channels. | en_US |
dc.format.extent | 514024 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Radiosynthesis | en_US |
dc.subject.other | Biomedical Engineering | en_US |
dc.subject.other | Receptor Binding | en_US |
dc.subject.other | Medical Law | en_US |
dc.subject.other | Autoradiogram | en_US |
dc.subject.other | Pharmacology/Toxicology | en_US |
dc.subject.other | Pharmacy | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | (±)L-iodo-MK-801 | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.title | Synthesis and Receptor Binding Studies of ( ± )l-Iodo-MK-801 | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pharmacy and Pharmacology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Nuclear Medicine, The University of Michigan Medical School, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Nuclear Medicine, The University of Michigan Medical School, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Nuclear Medicine, The University of Michigan Medical School, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Nuclear Medicine, The University of Michigan Medical School, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Nuclear Medicine, The University of Michigan Medical School, Ann Arbor, Michigan, 48109; Department of Nuclear Medicine, The University of Texas M.D. Anderson Cancer Center, Box 57, 1515 Holcombe Boulevard, Houston, Texas, 77030 | en_US |
dc.contributor.affiliationum | Department of Nuclear Medicine, The University of Michigan Medical School, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Nuclear Medicine, The University of Michigan Medical School, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 2548173 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41537/1/11095_2004_Article_306014.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1015912322247 | en_US |
dc.identifier.source | Pharmaceutical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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