Secretion of parathyroid hormone-related protein by bovine mammary cells in vitro
dc.contributor.author | Okada, Hiroyuki | en_US |
dc.contributor.author | Rosol, Thomas J. | en_US |
dc.contributor.author | Capen, Charles C. | en_US |
dc.contributor.author | McCauley, Laurie K. | en_US |
dc.contributor.author | Weckmann, Michelle T. | en_US |
dc.contributor.author | Schanbacher, Floyd L. | en_US |
dc.date.accessioned | 2006-09-08T19:25:36Z | |
dc.date.available | 2006-09-08T19:25:36Z | |
dc.date.issued | 1994-02 | en_US |
dc.identifier.citation | Okada, Hiroyuki; Schanbacher, Floyd L.; McCauley, Laurie K.; Capen, Charles C.; Weckmann, Michelle T.; Rosol, Thomas J.; (1994). "Secretion of parathyroid hormone-related protein by bovine mammary cells in vitro ." Journal of Bone and Mineral Metabolism 12(1): S157-S160. <http://hdl.handle.net/2027.42/41592> | en_US |
dc.identifier.issn | 0914-8779 | en_US |
dc.identifier.issn | 1435-5604 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41592 | |
dc.description.abstract | Mammary cells were isolated from lactating cows at 1 to 6 weeks after calving and evaluated for their ability to secrete PTHrP in vitro. The tissue was enzymatically digested to release glandular acini. The digested acini were cultured on thin (1.0 mm) or thick (2.5 mm) layers of collagen. The cultures containing thick collagen were detached and allowed to contract on day 6. The culture medium consisted of M199 with prolation (8 µg/ml), insulin (5 µg/ml), cortisol (5 µg/ml), and fetal bovine serum (15%). PTHrP production was measured by N-terminal RIA and bioassay (stimulation of adenylate cyclase in the ROS 17/2.8 cell line). Medium was collected at 2-day intervals for 14 days. The cells reached confluence at 4–6 days. PTHrP production was low at day 2 (<0.5 ng/ml), but increased to peak production (2–4 ng/ml) at approximately day 6–8 of culture and remained constant until day 14. Immunoreactive and bioactive PTHrP levels in the culture medium correlated well. The cultures produced high levels of lactoferrin (500 to 3000 ng/ml) and low levels of α s1 -casein (14 to 77 ng/ml). | en_US |
dc.format.extent | 299791 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Japanese Society of Bone Metabolism Research | en_US |
dc.subject.other | Metabolic Diseases | en_US |
dc.subject.other | Mammary Gland | en_US |
dc.subject.other | Internal Medicine | en_US |
dc.subject.other | Lactation | en_US |
dc.subject.other | Orthopedics | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | PTHrP | en_US |
dc.title | Secretion of parathyroid hormone-related protein by bovine mammary cells in vitro | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Periodontics/Prevention & Geriatrics, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Veterinary Pathobiology, The Ohio State University, Columbus, OH, USA | en_US |
dc.contributor.affiliationother | Department of Veterinary Pathobiology, The Ohio State University, Columbus, OH, USA | en_US |
dc.contributor.affiliationother | Department of Veterinary Pathobiology, The Ohio State University, Columbus, OH, USA | en_US |
dc.contributor.affiliationother | Department of Dairy Science, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, USA | en_US |
dc.contributor.affiliationother | Department of Veterinary Pathobiology, The Ohio State University, Columbus, OH, USA; Rakuno Gakuen University Dept. of Veterinaty Pathology, 582, Bunkyodai-Midorimachi, 069, Ebetsu Hokkaido, Japan | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41592/1/774_2006_Article_BF02375695.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF02375695 | en_US |
dc.identifier.source | Journal of Bone and Mineral Metabolism | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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