Enzyme-linked flow-injection immunoassay using immobilized secondary antibodies
dc.contributor.author | Lee, Ing Huei | en_US |
dc.contributor.author | Meyerhoff, Mark E. | en_US |
dc.date.accessioned | 2006-09-08T19:27:44Z | |
dc.date.available | 2006-09-08T19:27:44Z | |
dc.date.issued | 1988-01 | en_US |
dc.identifier.citation | Lee, Ing Huei; Meyerhoff, Mark E.; (1988). "Enzyme-linked flow-injection immunoassay using immobilized secondary antibodies." Mikrochimica Acta 96 (1-6): 207-221. <http://hdl.handle.net/2027.42/41625> | en_US |
dc.identifier.issn | 0026-3672 | en_US |
dc.identifier.issn | 1436-5073 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41625 | |
dc.description.abstract | A fast, non-equilibrium enzyme-linked flow-injection immunoassay (FIIA) system using an immobilized secondary-antibody reactor is described. The assay method is based on the competition between the enzyme-labeled antigen and analyte (unlabeled antigen) for a limited amount of soluble primary-antibody binding sites. This mixture is then introduced via flow-injection into the secondaryantibody reactor. The reactor bound enzyme activity, as measured by flowing an appropriate substrate solution through the reactor, is inversely proportional to the concentration of free analyte in the sample. By using non-equilibrium conditions, a single assay takes a total time of 13 min or less including regeneration of the reactor. To illustrate the application of this system, theophylline and insulin were chosen as model hapten and macromolecule analytes, respectively. Preliminary studies suggest that the new FIIA system is suitable for determining theophylline in serum with acceptable accuracy and precision. | en_US |
dc.format.extent | 983760 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Flow Injection Analysis | en_US |
dc.subject.other | Inorganic Chemistry | en_US |
dc.subject.other | Analytical Chemistry | en_US |
dc.subject.other | Insulin | en_US |
dc.subject.other | Monitoring/Environmental Analysis/Environmental Ecotoxicology | en_US |
dc.subject.other | Competitive Binding Assays | en_US |
dc.subject.other | Ion-selective Electrode | en_US |
dc.subject.other | Characterization and Evaluation Materials | en_US |
dc.subject.other | Physical Chemistry | en_US |
dc.subject.other | Theophylline | en_US |
dc.subject.other | Chemistry | en_US |
dc.title | Enzyme-linked flow-injection immunoassay using immobilized secondary antibodies | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, The University of Michigan, 48109-1055, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, The University of Michigan, 48109-1055, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41625/1/604_2005_Article_BF01236105.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01236105 | en_US |
dc.identifier.source | Mikrochimica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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