Biological characteristics of a cold-adapted influenza A virus mutation residing on a polymerase gene
dc.contributor.author | Tosaka, A. | en_US |
dc.contributor.author | Maassab, Hunein F. | en_US |
dc.contributor.author | Ishida, N. | en_US |
dc.contributor.author | Odagiri, Takato | en_US |
dc.date.accessioned | 2006-09-08T19:31:59Z | |
dc.date.available | 2006-09-08T19:31:59Z | |
dc.date.issued | 1986-03 | en_US |
dc.identifier.citation | Odagiri, T.; Tosaka, A.; Ishida, N.; Maassab, H. F.; (1986). "Biological characteristics of a cold-adapted influenza A virus mutation residing on a polymerase gene." Archives of Virology 88 (1-2): 91-104. <http://hdl.handle.net/2027.42/41692> | en_US |
dc.identifier.issn | 1432-8798 | en_US |
dc.identifier.issn | 0304-8608 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41692 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2420313&dopt=citation | en_US |
dc.description.abstract | The biological function of a cold-adapted (ca) mutation residing on the PB2 gene of an influenza A/Ann Arbor/6/60 (A/AA/6/60) ca variant virus in the viral replication cycle at 25° C was studied. The viral polypeptide synthesis of A/AA/6/60 ca variant at 25° C was evident approximately 6 hours earlier than the wild type (wt) virus and yielded twice as many products. The quantitative analysis of viral complementary RNA (cRNA), synthesized in the presence of cycloheximide, revealed that A/AA/6/60 ca variant and a single gene reassortant that contains only the PB2 gene of the ca variant with remaining genes of the wt virus produced equal amount of cRNA at 25° and 33° C, which was an amount approximately four fold greater than the wt virus' cRNA synthesized at 25° C. These results strongly suggest that the ca mutation residing on the PB2 gene of A/AA/6/60 ca variant affects the messenger RNA synthesis at 25° C in the primary transcription. | en_US |
dc.format.extent | 1379868 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Medical Microbiology | en_US |
dc.subject.other | Virology | en_US |
dc.subject.other | Infectious Diseases | en_US |
dc.title | Biological characteristics of a cold-adapted influenza A virus mutation residing on a polymerase gene | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Bacteriology, Tohoku University, School of Medicine, Sendai, Japan | en_US |
dc.contributor.affiliationother | Department of Bacteriology, Tohoku University, School of Medicine, Sendai, Japan; Department of Virology, Jichi Medical School, Minamikawachi-machi, Kawachi-gun, 329-04, Tochigi-ken, Japan | en_US |
dc.contributor.affiliationother | Department of Bacteriology, Tohoku University, School of Medicine, Sendai, Japan | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 2420313 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41692/1/705_2005_Article_BF01310893.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01310893 | en_US |
dc.identifier.source | Archives of Virology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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