Hydrogen peroxide generation by mitochondria isolated from regionally ischemic and nonischemic dog myocardium
dc.contributor.author | Gallagher, Kim P. | en_US |
dc.contributor.author | Adkins, Steve | en_US |
dc.contributor.author | Shlafer, Marshal | en_US |
dc.date.accessioned | 2006-09-08T19:35:45Z | |
dc.date.available | 2006-09-08T19:35:45Z | |
dc.date.issued | 1990-07 | en_US |
dc.identifier.citation | Shlafer, M.; Gallagher, K. P.; Adkins, S.; (1990). "Hydrogen peroxide generation by mitochondria isolated from regionally ischemic and nonischemic dog myocardium." Basic Research in Cardiology 85(4): 318-329. <http://hdl.handle.net/2027.42/41749> | en_US |
dc.identifier.issn | 0300-8428 | en_US |
dc.identifier.issn | 1435-1803 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41749 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2241765&dopt=citation | en_US |
dc.description.abstract | We occluded the left anterior descending coronary artery of anesthetized, open-chest dogs, for 1 or 2 h. Some hearts were reperfused for 1 h after 1 h of ischemia. We isolated mitochondria from the central ischemic zone (CIZ) and a surrounding nonischemic zone (NIZ) of the left ventricle, and assayed H 2 O 2 production using a horseradish peroxidase-dual wavelength spectrophotometric technique. Mitochondria, studied in the absence of exogenous respiratory chain inhibitors, generated H 2 O 2 during State 4 respiration with succinate as the substrate. NIZ mitochondria in all groups produced ca. 1.5 nmols H 2 O 2 /min/mg protein (no significant differences between groups). The State 4 O 2 consumption rates of NIZ mitochondria from hearts subjected to 1 h ischemia plus reperfusion, or 2 h of ischemia (ca. 30 nmols/min/mg) were significantly higher than that of NIZ mitochondria of hearts subjected to only 1 h of ischemia (23 nmols/min/mg). Thus, the ratio between H 2 O 2 produced and State 4 O 2 consumption fell from 6.5% to 5%. Mitochondria from all CIZ samples had State 4 O 2 consumption rates that were not different from corresponding NIZ values. However CIZ mitochondria of hearts subjected to 1 h ischemia without reperfusion produced less H 2 O 2 (1.1±0.1 nmols/min/mg), and had a slightly reduced H 2 O 2 /O 2 ratio (4.4±0.7%), compared with their NIZ samples (1.5±0.1 nmols/min/mg; 5.3%). Reperfusion after 1 h of ischemia abolished these regional differences. The CIZ mitochondria from hearts subjected to 2 h ischemia produced only 0.75±0.22 nmols H 2 O 2 /min/mg (2.5% of State 4 O 2 consumption). These values were 50% of corresponding NIZ values, and were significantly less than for any other group or tissue region. If similar phenomena occur in conscious animals subjected to incomplete regional ischemia, especially of relatively brief duration or if accompanied by reduced intracellular defenses against oxidants such as H 2 O 2 , they suggest that mitochondria persist as H 2 O 2 sources and so may contribute to the oxidant load and myocardial dysfunction. | en_US |
dc.format.extent | 764437 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Steinkopff-Verlag; Dr. Dietrich Steinkopff Verlag ; Springer Science+Business Media | en_US |
dc.subject.other | Cardiology | en_US |
dc.subject.other | I Schemia (Regional) | en_US |
dc.subject.other | M Itochondria | en_US |
dc.subject.other | H Ydrogen Peroxide | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | H Eart | en_US |
dc.subject.other | R Eperfusion | en_US |
dc.title | Hydrogen peroxide generation by mitochondria isolated from regionally ischemic and nonischemic dog myocardium | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, The University of Michigan Medical School, Box M-035, 48109-0626, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Pharmacology, The University of Michigan Medical School, Box M-035, 48109-0626, Ann Arbor, Michigan, USA; Department of Surgery (Section of Thoracic Surgery), The University of Michigan Medical School, Box M-035, 48109-0626, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Surgery (Section of Thoracic Surgery), The University of Michigan Medical School, Box M-035, 48109-0626, Ann Arbor, Michigan, USA; Department of Physiology, The University of Michigan Medical School, Box M-035, 48109-0626, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 2241765 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41749/1/395_2005_Article_BF01907125.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01907125 | en_US |
dc.identifier.source | Basic Research in Cardiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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