Do Femtonewton Forces Affect Genetic Function? A Review
dc.contributor.author | Blumberg, Seth | en_US |
dc.contributor.author | Pennington, Matthew W. | en_US |
dc.contributor.author | Meiners, Jens-Christian | en_US |
dc.date.accessioned | 2006-09-08T19:40:06Z | |
dc.date.available | 2006-09-08T19:40:06Z | |
dc.date.issued | 2006-03-29 | en_US |
dc.identifier.citation | Blumberg, Seth; Pennington, Matthew W.; Meiners, Jens-Christian; (2006). "Do Femtonewton Forces Affect Genetic Function? A Review." Journal of Biological Physics (): 1-23. <http://hdl.handle.net/2027.42/41816> | en_US |
dc.identifier.issn | 0092-0606 | en_US |
dc.identifier.issn | 1573-0689 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41816 | |
dc.description.abstract | Protein-Mediated DNA looping is intricately related to gene expression. Therefore any mechanical constraint that disrupts loop formation can play a significant role in gene regulation. Polymer physics models predict that less than a piconewton of force may be sufficient to prevent the formation of DNA loops. Thus, it appears that tension can act as a molecular switch that controls the much larger forces associated with the processive motion of RNA polymerase. Since RNAP can exert forces over 20 pN before it stalls, a ‘substrate tension switch’ could offer a force advantage of two orders of magnitude. Evidence for such a mechanism is seen in recent in vitro micromanipulation experiments. In this article we provide new perspective on existing theory and experimental data on DNA looping in vitro and in vivo . We elaborate on the connection between tension and a variety of other intracellular mechanical constraints including sequence specific curvature and supercoiling. In the process, we emphasize that the richness and versatility of DNA mechanics opens up a whole new paradigm of gene regulation to explore. | en_US |
dc.format.extent | 277227 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media, Inc. | en_US |
dc.subject.other | DNA | en_US |
dc.subject.other | Mechanics | en_US |
dc.subject.other | Looping | en_US |
dc.subject.other | Tension | en_US |
dc.title | Do Femtonewton Forces Affect Genetic Function? A Review | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Physics | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Physics and Biophysics Research Division, Randall Laboratory, University of Michigan, Ann Arbor, MI, 48109-1120, USA | en_US |
dc.contributor.affiliationum | Department of Physics and Biophysics Research Division, Randall Laboratory, University of Michigan, Ann Arbor, MI, 48109-1120, USA | en_US |
dc.contributor.affiliationum | Department of Physics and Biophysics Research Division, Randall Laboratory, University of Michigan, Ann Arbor, MI, 48109-1120, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41816/1/10867_2005_Article_9002.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s10867-005-9002-8 | en_US |
dc.identifier.source | Journal of Biological Physics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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