Effect of slow release IL-12 and IL-10 on inflammation, local macrophage function and the regional lymphoid response during mycobacterial (Th1) and schistosomal (Th2) antigen-elicited pulmonary granuloma formation
dc.contributor.author | Kunkel, Steven L. | en_US |
dc.contributor.author | Chensue, Stephen W. | en_US |
dc.contributor.author | Warmington, K. S. | en_US |
dc.contributor.author | Ruth, Jeffrey H. | en_US |
dc.date.accessioned | 2006-09-08T19:40:13Z | |
dc.date.available | 2006-09-08T19:40:13Z | |
dc.date.issued | 1997-03 | en_US |
dc.identifier.citation | Chensue, S. W.; Warmington, K.; Ruth, J. H.; Kunkel, S. L.; (1997). "Effect of slow release IL-12 and IL-10 on inflammation, local macrophage function and the regional lymphoid response during mycobacterial (Th1) and schistosomal (Th2) antigen-elicited pulmonary granuloma formation." Inflammation Research 46(3): 86-92. <http://hdl.handle.net/2027.42/41818> | en_US |
dc.identifier.issn | 1023-3830 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41818 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9098720&dopt=citation | en_US |
dc.description.abstract | Objective and Design: This study examines the local and regional effects of exogenously administered interleukins 10 (IL-10) and 12 (IL-12) on pulmonary granulomas mediated by Th1/type 1-(IFN-<gamma>) and Th2/type 2-(IL-4, IL-5) cytokines. ¶ Materials and Treatments: Granulomas (GR) were induced in presensitized CBA mice by embolization of beads coated with Mycobacteria tuberculosis or Schistosoma mansoni egg antigens. Before challenge, osmotic pumps distributing IL-10 or IL-12 (50 <mu>g/kg/day) were implanted intraperitoneally, then GR and draining lymph nodes were examined 4 days. ¶ Methods: GR sizes and composition were determined by morphometry and differential analysis. Isolated GR macrophages and draining lymph nodes were assessed for cytokine production by ELISA. ¶ Results: IL-10 did not effect GR sizes but reduced neutrophils in type 1 GR. IL-12 minimally reduced type 1 GR but decreased the type 2 lesion by up to 70%, primarily curtailing eosinophils. Type 2 GR macrophages were unaffected but type 1 were impaired by IL-10. Conversely, type 1 GR macrophages were more resistant to IL-12 while type 2 showed enhanced IL-10, IL-12 and TNF, but reduced MCP-1 production. In lymph nodes, IL-10 caused paradoxical effects, enhancing IFN-<gamma> in the type 1 and decreasing Th2 cytokines in the type 2 response. Exogenous IL-12 profoundly augmented IFN-<gamma> and abrogated type 2 cytokines while inhibiting intrinsic IL-12 production in lymph nodes. ¶ Conclusion: These findings provide novel information regarding cytokine regulation and the effects of systemic cytokine therapy. | en_US |
dc.format.extent | 190534 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Birkhäuser Verlag; Birkhäuser Verlag, Basel, ; Springer Science+Business Media | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | Key Words: Interleukin 10 — Interleukin 12 — Granuloma — Immunotherapy | en_US |
dc.title | Effect of slow release IL-12 and IL-10 on inflammation, local macrophage function and the regional lymphoid response during mycobacterial (Th1) and schistosomal (Th2) antigen-elicited pulmonary granuloma formation | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pharmacy and Pharmacology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, 1301 Catherine Rd., Ann Arbor, MI 48109, USA, US, | en_US |
dc.contributor.affiliationother | Department of Pathology and Laboratory Medicine, Veterans Affairs Medical Center, 2215 Fuller Rd., Ann Arbor, MI 48105, USA, US, | en_US |
dc.contributor.affiliationother | Department of Pathology and Laboratory Medicine, Veterans Affairs Medical Center, 2215 Fuller Rd., Ann Arbor, MI 48105, USA, US, | en_US |
dc.contributor.affiliationother | Department of Pathology and Laboratory Medicine, Veterans Affairs Medical Center, 2215 Fuller Rd., Ann Arbor, MI 48105, USA, US, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 9098720 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41818/1/11-46-3-86_70460086.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s000110050101 | en_US |
dc.identifier.source | Inflammation Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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