Characterization of rat lung ICAM-1
dc.contributor.author | Schimmer, R. C. | en_US |
dc.contributor.author | Beck-Schimmer, B. | en_US |
dc.contributor.author | Ward, Peter A. | en_US |
dc.contributor.author | Friedl, Hans P. | en_US |
dc.contributor.author | Flory, C. M. | en_US |
dc.contributor.author | Schmal, H. | en_US |
dc.contributor.author | Pasch, T. | en_US |
dc.date.accessioned | 2006-09-08T19:40:21Z | |
dc.date.available | 2006-09-08T19:40:21Z | |
dc.date.issued | 1998-07 | en_US |
dc.identifier.citation | Beck-Schimmer, B.; Schimmer, R. C.; Schmal, H.; Flory, C. M.; Friedl, H. P.; Pasch, T.; Ward, P. A.; (1998). "Characterization of rat lung ICAM-1." Inflammation Research 47(7): 308-315. <http://hdl.handle.net/2027.42/41820> | en_US |
dc.identifier.issn | 1023-3830 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41820 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9719495&dopt=citation | en_US |
dc.description.abstract | Objective and Design: We expressed soluble rat ICAM-1, generated a polyclonal anti-ICAM-1 antibody, and studied ICAM-1 upregulation in lung inflammatory conditions. Bacterial and baculovirus expression systems were employed.¶ Material: 250 g adult, male Long Evans rats were used. For in vitro studies, rat pulmonary artery endothelial cells (RPAEC), rat alveolar macrophages and aortic rings were stimulated (as described below) and evaluated for ICAM-1 expression.¶ Treatment: RPAEC and macrophages were stimulated with lipopolysaccharide (LPS) and recombinant murine tumour necrosis factor α (TNFα). In vivo immunoglobulin G (IgG) immune complex-induced lung injury was employed.¶ Methods: Enzyme-linked immunoassay (ELISA) Western and Northern blot analyses and immunohistochemical evaluations were performed. All experiments were done at least in duplicate. Data were analyzed by two-tailed Student’s t-test.¶ Results: ICAM-1 expression of RPAEC was time- and dose-dependent, peaking at 6 h after LPS-stimulation. LPS and TNFα each enhanced ICAM-1 expression on alveolar macrophages (reaching a maximum at 2 h). In IgG immune complex-induced lung injury, ICAM-1 mRNA isolated from whole lung peaked at 4 h, while lung ICAM-1 protein peaked at 6 h.¶ Conclusions: Quantitation of ICAM-1 expression in vitro and in vivo suggests that ICAM-1 plays a central role in two lung inflammatory models. Furthermore, lung ICAM-1 upregulation involves at least two cell types: vascular endothelial cells and alveolar macrophages. | en_US |
dc.format.extent | 607925 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Birkhäuser Verlag; Birkhäuser Verlag, Basel, ; Springer Science+Business Media | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | Key Words: Rat ICAM-1 — Anti-ICAM-1 — Protein Expression — Lung Inflammation | en_US |
dc.title | Characterization of rat lung ICAM-1 | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pharmacy and Pharmacology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, 1301 Catherine Road, Ann Arbor, MI 48109-0602 USA, Fax +1 734 763 4782, e-mail: pward@umich.edu, US, | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, 1301 Catherine Road, Ann Arbor, MI 48109-0602 USA, Fax +1 734 763 4782, e-mail: pward@umich.edu, US, | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, 1301 Catherine Road, Ann Arbor, MI 48109-0602 USA, Fax +1 734 763 4782, e-mail: pward@umich.edu, US, | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, 1301 Catherine Road, Ann Arbor, MI 48109-0602 USA, Fax +1 734 763 4782, e-mail: pward@umich.edu, US, | en_US |
dc.contributor.affiliationother | Department of Surgery, University of Zurich Medical School, Rämistr. 100, CH-8091 Zurich, Switzerland, CH, | en_US |
dc.contributor.affiliationother | Department of Immunopathology, Parke Davis Pharmaceutical Research, Division of Warner Lambert Company, 2800 Plymouth Road, Ann Arbor, MI 48105, USA, US, | en_US |
dc.contributor.affiliationother | Institute for Anesthesiology, University of Zurich Medical School, Rämistr. 100, CH-8091 Zurich, Switzerland, CH, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 9719495 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41820/1/11-47-7-308_80470308.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s000110050334 | en_US |
dc.identifier.source | Inflammation Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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