Stem cell factor and IgE-stimulated murine mast cells produce chemokines (CCL2, CCL17, CCL22) and express chemokine receptors
dc.contributor.author | Oliveira, S. H. P. | en_US |
dc.contributor.author | Lukacs, Nicholas W. | en_US |
dc.date.accessioned | 2006-09-08T19:40:36Z | |
dc.date.available | 2006-09-08T19:40:36Z | |
dc.date.issued | 2001-03 | en_US |
dc.identifier.citation | Oliveira, S.H.P.; Lukacs, N.W.; (2001). "Stem cell factor and IgE-stimulated murine mast cells produce chemokines (CCL2, CCL17, CCL22) and express chemokine receptors." Inflammation Research 50(3): 168-174. <http://hdl.handle.net/2027.42/41824> | en_US |
dc.identifier.issn | 1023-3830 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41824 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11339505&dopt=citation | en_US |
dc.description.abstract | Objective and design: In the present study we investigated the effect of SCF and/or IgE on histamine, TNF- α and chemokines released from bone marrow-derived mast cells (BMMC) as well as chemokine receptor expression.¶ Material and methods: BMMC were derived from femoral bone marrow of CBA/J mice. The purity of BMMC was >98%percnt; after 3 weeks. BMMC (2.5 × 10 6 cells/well) were incubated in the presence or absence of either SCF, IgE plus DNP or a combination of SCF and IgE for 6 and 18 h. Cell-free supernatants were recovered to measure CC chemokines, TNF- α and histamine release utilizing ELISA assays. CC chemokine family receptors were detected by RT-PCR analysis, and confirmed using functional chemotactic assays.¶ Results: Histamine levels were comparable between SCF and IgE stimulated cells, whereas TNF- α production was significantly greater after IgE compared to SCF stimulation. SCF and/or IgE-stimulated BMMC released CC chemokines, CCL22 (MDC), CCL17 (TARC) and CCL2 (MCP-1). Increased mRNA expression of CCR1, CCR2, CCR3, and CCR5 was detected in SCF and IgE-stimulated BMMCs. Functional chemotactic assays confirmed the expression data.¶ Conclusion: SCF and IgE can up-regulate the expression of chemokines and chemokine receptors on mast cells. Thus, SCF may play a significant role in their activation and inflammation during allergic responses.¶ | en_US |
dc.format.extent | 148111 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Birkhäuser Verlag; Birkhäuser Verlag, ; Springer Science+Business Media | en_US |
dc.subject.other | Key Words: SCF - Mast Cells - CC Chemokine - CC Chemokine Receptor - Histamine - TNF-α | en_US |
dc.subject.other | Legacy | en_US |
dc.title | Stem cell factor and IgE-stimulated murine mast cells produce chemokines (CCL2, CCL17, CCL22) and express chemokine receptors | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pharmacy and Pharmacology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan Medical School - Department of Pathology, 1301 Catherinest, Ann Arbor, MI 48109-0602, Fax: 734-764-2397, e-mail: nlukacs@umich.edu , US, | en_US |
dc.contributor.affiliationum | University of Michigan Medical School - Department of Pathology, 1301 Catherinest, Ann Arbor, MI 48109-0602, Fax: 734-764-2397, e-mail: nlukacs@umich.edu , US, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 11339505 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41824/1/11-50-3-168_10500168.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s000110050741 | en_US |
dc.identifier.source | Inflammation Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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