Dichloromethane and trichloroethylene inhibition of methane oxidation by the membrane-associated methane monooxygenase of Methylosinus trichosporium OB3b
dc.contributor.author | Semrau, Jeremy D. | en_US |
dc.contributor.author | DiSpirito, Alan A. | en_US |
dc.contributor.author | Lontoh, Sonny | en_US |
dc.date.accessioned | 2006-09-08T19:46:35Z | |
dc.date.available | 2006-09-08T19:46:35Z | |
dc.date.issued | 1999-04 | en_US |
dc.identifier.citation | Lontoh, Sonny; DiSpirito, Alan A.; Semrau, J. D.; (1999). "Dichloromethane and trichloroethylene inhibition of methane oxidation by the membrane-associated methane monooxygenase of Methylosinus trichosporium OB3b." Archives of Microbiology 171(5): 301-308. <http://hdl.handle.net/2027.42/41917> | en_US |
dc.identifier.issn | 0302-8933 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41917 | |
dc.description.abstract | Whole-cell assays were used to measure the effect of dichloromethane and trichloroethylene on methane oxidation by Methylosinus trichosporium OB3b synthesizing the membrane-associated or particulate methane monooxygenase (pMMO). For M. trichosporium OB3b grown with 20 μM copper, no inhibition of methane oxidation was observed in the presence of either dichloromethane or trichloroethylene. If 20 mM formate was added to the reaction vials, however, methane oxidation rates increased and inhibition of methane oxidation was observed in the presence of dichloromethane and trichloroethylene. In the presence of formate, dichloromethane acted as a competitive inhibitor, while trichloroethylene acted as a noncompetitive inhibitor. The finding of noncompetitive inhibition by trichloroethylene was further examined by measuring the inhibition constants K iE and K iES . These constants suggest that trichloroethylene competes with methane at some sites, although it can bind to others if methane is already bound. Whole-cell oxygen uptake experiments for active and acetylene-treated cells also showed that provision of formate could stimulate both methane and trichloroethylene oxidation and that trichloroethylene did not affect formate dehydrogenase activity. The finding that different chlorinated hydrocarbons caused different inhibition patterns can be explained by either multiple substrate binding sites existing in pMMO or multiple forms of pMMO with different activities. The whole-cell analysis performed here cannot distinguish between these models, and further work should be done on obtaining active preparations of the purified pMMO. | en_US |
dc.format.extent | 90769 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag Berlin Heidelberg | en_US |
dc.subject.other | Trichloroethylene | en_US |
dc.subject.other | Inhibition | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | Key Words Particulate Methane Monooxygenase | en_US |
dc.subject.other | Dichloromethane | en_US |
dc.title | Dichloromethane and trichloroethylene inhibition of methane oxidation by the membrane-associated methane monooxygenase of Methylosinus trichosporium OB3b | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Civil and Environmental Engineering, The University of Michigan, 1351 Beal Avenue, Ann Arbor, MI 48109–2125, USA e-mail: jsemrau@engin.umich.edu Tel. +1-734-7646487; Fax +1-734-7632275, US, | en_US |
dc.contributor.affiliationum | Department of Civil and Environmental Engineering, The University of Michigan, 1351 Beal Avenue, Ann Arbor, MI 48109–2125, USA e-mail: jsemrau@engin.umich.edu Tel. +1-734-7646487; Fax +1-734-7632275, US, | en_US |
dc.contributor.affiliationother | Department of Microbiology, Iowa State University, Ames, IA 50011–3211, USA, US, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41917/1/203-171-5-301_91710301.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s002030050714 | en_US |
dc.identifier.source | Archives of Microbiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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