Gene gun application in the generation of effector T cells for adoptive immunotherapy
dc.contributor.author | Tanigawa, Keishi | en_US |
dc.contributor.author | Sun, Rong | en_US |
dc.contributor.author | Chang, Alfred E. | en_US |
dc.contributor.author | Yu, Hua | en_US |
dc.contributor.author | Nickoloff, Brian J. | en_US |
dc.date.accessioned | 2006-09-08T19:57:12Z | |
dc.date.available | 2006-09-08T19:57:12Z | |
dc.date.issued | 2000-01 | en_US |
dc.identifier.citation | Tanigawa, Keishi; Yu, Hua; Sun, Rong; Nickoloff, Brian J.; Chang, Alfred E.; (2000). "Gene gun application in the generation of effector T cells for adoptive immunotherapy." Cancer Immunology, Immunotherapy 48(11): 635-643. <http://hdl.handle.net/2027.42/42084> | en_US |
dc.identifier.issn | 0340-7004 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42084 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10663611&dopt=citation | en_US |
dc.description.abstract | We utilized the gene gun to transfect subcutaneous D5 melanoma and MT-901 mammary carcinoma tumors in situ with a granulocyte/macrophage-colony-stimulating factor (GM-CSF) plasmid complexed to gold particles. There was diminished tumor growth following bombardment with GM-CSF plasmid, which was apparent only during the period of administration. Transgenic GM-CSF was produced by the skin overlying the tumors and not by the tumors themselves. GM-CSF plasmid bombardment resulted in increased cell yields within tumor-draining lymph nodes (TDLN) with at least a 12-fold increase in the percentage of dendritic cells (8.9%) compared to controls (0.7%). Secondarily activated TDLN cells from animals transfected with GM-CSF demonstrated enhanced cytokine release (interferon γ, GM-CSF and interleukin-10) in response to tumor stimulator cells compared to controls, and had an increased capacity to mediate tumor regression in adoptive immunotherapy. There was a small, but detectable, non-specific immune adjuvant effect observed with gold particle bombardment alone, which was less than with GM-CSF plasmid. The adjuvant effect of GM-CSF plasmid required peri-tumoral transgene expression since gene bombardment away from the tumor was ineffective. | en_US |
dc.format.extent | 503440 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag Berlin Heidelberg | en_US |
dc.subject.other | Gene Therapy | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | Key Words T Cells | en_US |
dc.subject.other | Adoptive Immunotherapy | en_US |
dc.subject.other | Vaccines | en_US |
dc.title | Gene gun application in the generation of effector T cells for adoptive immunotherapy | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Surgical Oncology, University of Michigan, 3302 Cancer Center, 1500 E. Medical Center Drive, Ann Arbor, MI 48109-0932, USA e-mail: aechang@umich.edu Tel.: +1-734-936-4392 Fax: +1-734-647-9647, US | en_US |
dc.contributor.affiliationum | Division of Surgical Oncology, University of Michigan, 3302 Cancer Center, 1500 E. Medical Center Drive, Ann Arbor, MI 48109-0932, USA e-mail: aechang@umich.edu Tel.: +1-734-936-4392 Fax: +1-734-647-9647, US | en_US |
dc.contributor.affiliationum | Division of Surgical Oncology, University of Michigan, 3302 Cancer Center, 1500 E. Medical Center Drive, Ann Arbor, MI 48109-0932, USA e-mail: aechang@umich.edu Tel.: +1-734-936-4392 Fax: +1-734-647-9647, US | en_US |
dc.contributor.affiliationother | Department of Pathology, Loyola University Medical Center, Maywood, IL 00153, USA, US | en_US |
dc.contributor.affiliationother | H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, College of Medicine, Tampa, FL 33612, USA, US | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 10663611 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42084/1/262-48-11-635_00480635.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s002620050012 | en_US |
dc.identifier.source | Cancer Immunology, Immunotherapy | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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