Evaluation of probiotic treatment in a neonatal animal model
dc.contributor.author | Lee, D. J. | en_US |
dc.contributor.author | Drongowski, Robert A. | en_US |
dc.contributor.author | Harmon, Carroll M. | en_US |
dc.contributor.author | Coran, Arnold G. | en_US |
dc.date.accessioned | 2006-09-08T20:03:49Z | |
dc.date.available | 2006-09-08T20:03:49Z | |
dc.date.issued | 2000-05 | en_US |
dc.identifier.citation | Lee, D. J.; Drongowski, R. A.; Coran, A. G.; Harmon, C. M.; (2000). "Evaluation of probiotic treatment in a neonatal animal model." Pediatric Surgery International 16(4): 237-242. <http://hdl.handle.net/2027.42/42187> | en_US |
dc.identifier.issn | 0179-0358 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42187 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10898221&dopt=citation | en_US |
dc.description.abstract | The clinical use of probiotic agents such as enteral Lactobacillus to enhance intestinal defense against potential luminal pathogens has been tested in vivo; however, an understanding of the mechanisms responsible for the observed protection is lacking. The purpose of this study was to evaluate the effects of Lactobacillus on bacterial translocation (BT) in a neonatal animal model. Newborn New Zealand white rabbit pups were enterally fed a 10% Formulac solution inoculated with or without a 10 8 suspension of ampicillin-resistant Escherichia coli K1 ( E. coli K1A) and/or Lactobacillus casei GG ( Lacto GG). Pups received either no bacteria (n = 10), Lacto GG (n = 8), E. coli K1A (n = 26), or a combination of Lacto GG and E. coli K1A (n = 33). On day 3, representative tissue specimens from the mesenteric lymph nodes (MLN), spleen (SPL), and liver (LIV) were aseptically harvested in addition to a small-bowel (SB) sample that was rinsed to remove luminal contents. The specimens were then cultured in organism-specific media. Statistical analysis was by one-way ANOVA with P values less than 0.05 considered significant. Neonatal rabbits receiving Lacto GG-supplemented formula exhibited a 25% decrease ( P < 0.05) in small-bowel colonization by E. coli K1A. In addition, Lacto GG decreased the frequency of extraintestinal BT by 46% ( P < 0.05), 61% ( P < 0.05), and 23%, respectively, in the MLN, SPL, and LIV. We have shown that enterally-administered Lacto GG decreases the frequency of E. coli K1A translocation in a neonatal rabbit model. These results may have significant implications for the treatment of BT and sepsis in the human neonate and provide a model for further studies. | en_US |
dc.format.extent | 113065 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag Berlin Heidelberg | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | Neonate | en_US |
dc.subject.other | Probiotics | en_US |
dc.subject.other | Key Words Bacterial Translocation | en_US |
dc.title | Evaluation of probiotic treatment in a neonatal animal model | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pediatrics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, US | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, US | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, US | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, US | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 10898221 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42187/1/383-16-4-237_00160237.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s003830050736 | en_US |
dc.identifier.source | Pediatric Surgery International | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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