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The effect of phospholipase A2 on bacterial translocation in a cell culture model

dc.contributor.authorSawai, Toshioen_US
dc.contributor.authorDrongowski, Robert A.en_US
dc.contributor.authorCoran, Arnold G.en_US
dc.contributor.authorUsui, N.en_US
dc.contributor.authorHarmon, Carroll M.en_US
dc.contributor.authorDwaihy, J.en_US
dc.contributor.authorAbe, Akiraen_US
dc.date.accessioned2006-09-08T20:03:53Z
dc.date.available2006-09-08T20:03:53Z
dc.date.issued2000-05en_US
dc.identifier.citationSawai, T.; Usui, N.; Dwaihy, J.; Drongowski, R. A.; Abe, A.; Coran, A. G.; Harmon, C. M.; (2000). "The effect of phospholipase A2 on bacterial translocation in a cell culture model." Pediatric Surgery International 16(4): 262-266. <http://hdl.handle.net/2027.42/42188>en_US
dc.identifier.issn0179-0358en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/42188
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10898226&dopt=citationen_US
dc.description.abstract The activity of phospholipase (PL)A 2 is elevated in the intestinal epithelia of patients with inflammatory bowel disease (IBD). Recently, we reported that lysophosphatidylcholine (L-PC), the PLA 2 hydrolysis product of phosphatidylcholine (PC), stimulates bacterial translocation (BT) in an enterocyte cell-culture model. These two observations stimulated us to examine the effects of extracellular PLA 2 on intestinal epithelial permeability. Human Caco-2 enterocytes were grown to confluence on porous filters in the apical chamber of a two-chamber cell-culture system. Monolayer integrity and tight-junction permeability were measured by dextran blue (DB) permeability and transepithelial electric resistance (TEER). Monolayers were treated with PC, L-PC, or PLA 2 with and without PC. The magnitude of BT was determined 2 h after treatment by adding Escherichia coli to the apical chamber followed by quantitatively culturing basal chamber samples. Thin-layer chromatography (TLC) was utilized to verify PLA 2 hydrolysis of PC to L-PC. Statistical analysis was performed by one-way analysis of variance. The magnitude of BT across monolayers pretreated with PLA 2  + PC significantly increased compared to either PC or PLA 2 (6.83 ± 0.069, 2.41 ± 0.46, and 3.06 ± 1.14 log10 colony forming units/ml, respectively, P  < 0.05). Absence of DB-permeability in any group confirmed monolayer integrity. TLC of PL samples harvested from the apical monolayer surface confirmed PC hydrolysis. PLA 2 mediates hydrolysis of PC to L-PC when both are applied to the apical surface of cultured enterocyte monolayers, resulting in increased BT and increased TEER with no damage to monolayer integrity. These observations may have implications in the pathogenesis and treatment strategies for IBD.en_US
dc.format.extent273805 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlag; Springer-Verlag Berlin Heidelbergen_US
dc.subject.otherLegacyen_US
dc.subject.otherKey Words Phospholipase A2en_US
dc.subject.otherTransepithelial Electrical Resistanceen_US
dc.subject.otherInflammatory Bowel Diseaseen_US
dc.subject.otherBacterial Translocationen_US
dc.subject.otherPhosphatidylcholineen_US
dc.subject.otherLysophosphatidylcholineen_US
dc.titleThe effect of phospholipase A2 on bacterial translocation in a cell culture modelen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelPediatricsen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumUniversity of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, USen_US
dc.contributor.affiliationumUniversity of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, USen_US
dc.contributor.affiliationumUniversity of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, USen_US
dc.contributor.affiliationumUniversity of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, USen_US
dc.contributor.affiliationumUniversity of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, USen_US
dc.contributor.affiliationumUniversity of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, USen_US
dc.contributor.affiliationumUniversity of Michigan Medical School, Section of Pediatric Surgery, F3970 Mott Children's Hospital, Ann Arbor, MI 48109-0245, USA, USen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid10898226en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/42188/1/383-16-4-262_00160262.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/s003830050741en_US
dc.identifier.sourcePediatric Surgery Internationalen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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