Topical all- trans retinoic acid (RA) induces an early, coordinated increase in RA-inducible skin-specific gene/psoriasin and cellular RA-binding protein II mRNA levels which precedes skin erythema
dc.contributor.author | Orfanos, Constantin E. | en_US |
dc.contributor.author | Voorhees, John J. | en_US |
dc.contributor.author | Tavakkol, Amir | en_US |
dc.contributor.author | Zouboulis, Christos C. | en_US |
dc.date.accessioned | 2006-09-08T20:05:15Z | |
dc.date.available | 2006-09-08T20:05:15Z | |
dc.date.issued | 1996-10 | en_US |
dc.identifier.citation | Zouboulis, C. C.; Voorhees, John J.; Orfanos, Constantin E.; Tavakkol, Amir; (1996). "Topical all- trans retinoic acid (RA) induces an early, coordinated increase in RA-inducible skin-specific gene/psoriasin and cellular RA-binding protein II mRNA levels which precedes skin erythema." Archives of Dermatological Research 288(11): 664-669. <http://hdl.handle.net/2027.42/42209> | en_US |
dc.identifier.issn | 0340-3696 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42209 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8931868&dopt=citation | en_US |
dc.description.abstract | Separation of specific and nonspecific “irritant” effects of topical all- trans retinoic acid (RA) is a key to understanding the mechanism of retinoid action in skin. Cellular RA-binding protein (CRABP) II has been found to be a marker of RA activity in human skin. We have also previously identified a skin-specific gene (RIS-1/psoriasin) which is rapidly induced in human skin treated with RA. Here we compared the kinetics and time-course of RIS-1 and CRABP II gene activation by RA, sodium lauryl sulfate (SLS), a classical irritant, and their vehicle (VH), using a quantitative reverse transcription polymerase chain reaction. RIS-1 and CRABP II were both expressed at very low levels in untreated normal human skin, and in RA-treated skin the kinetics and time course of RIS-1 and CRABP II mRNA induction were similar. Relative to VH-treated skin, RA induced RIS-1 mRNA levels within 6 h, which further increased to 6.4-fold by 24 h ( n = 4). Similarly, CRABP II mRNA levels increased from 2.6-fold at 6 h to 7.8-fold after 24 h. At 48 h the relative mRNA levels for both genes decreased towards the steady-state levels. Relative to SLS-treated skin, RIS-1 mRNA increased by 3.2-fold after 6 h and by 5.1-fold after 12 h ( n = 3). Also, a 2.6-fold higher CRABP II mRNA observed after 6 h increased to 6-fold after 12 h. After 24 and 48 h RA treatment the relative mRNA levels for both genes decreased towards the steady-state levels. RA-induced skin erythema was not obvious until 24 to 48 h. We conclude, therefore, that induction of RIS-1 and CRABP II mRNA levels by topical RA in human skin are early, coordinated molecular events which precede the clinical cutaneous erythematous response to RA. | en_US |
dc.format.extent | 161876 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag Berlin Heidelberg | en_US |
dc.subject.other | Human Skin | en_US |
dc.subject.other | Psoriasin Gene | en_US |
dc.subject.other | Key Words Retinoic Acid | en_US |
dc.subject.other | RIS-1/ | en_US |
dc.subject.other | Quantitative RT-PCR | en_US |
dc.subject.other | CRABP II Gene | en_US |
dc.subject.other | Legacy | en_US |
dc.title | Topical all- trans retinoic acid (RA) induces an early, coordinated increase in RA-inducible skin-specific gene/psoriasin and cellular RA-binding protein II mRNA levels which precedes skin erythema | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dermatology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Dermatology, University of Michigan Medical Center, 1910 A. Alfred Taubman Health Care Center, Ann Arbor, MI 48109-0314, USA, US | en_US |
dc.contributor.affiliationother | Department of Dermatology, University Medical Center Benjamin Franklin, The Free University of Berlin, Hindenburgdamm 30, D-12200 Berlin, Germany Tel. 49-30-84452769; Fax 49-30-84454262 e-mail: zoubbere@fub46.zedat.fu-berlin.de, DE | en_US |
dc.contributor.affiliationother | Colgate-Palmolive Company, Skin Clinical Investigations Group, 909 River Road, Piscataway, NJ 08855-1343, USA, US | en_US |
dc.contributor.affiliationother | Department of Dermatology, University Medical Center Benjamin Franklin, The Free University of Berlin, Hindenburgdamm 30, D-12200 Berlin, Germany Tel. 49-30-84452769; Fax 49-30-84454262 e-mail: zoubbere@fub46.zedat.fu-berlin.de, DE | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 8931868 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42209/1/403-288-11-664_62880664.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s004030050121 | en_US |
dc.identifier.source | Archives of Dermatological Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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