Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye: loss of LEDGF from nuclei of differentiating cells
dc.contributor.author | Reddy, Venkat N. | en_US |
dc.contributor.author | Singh, Dhirendra P. | en_US |
dc.contributor.author | Shinohara, Toshimichi | en_US |
dc.contributor.author | Zelenka, Peggy | en_US |
dc.contributor.author | Kubo, Eri | en_US |
dc.contributor.author | Chylack, Leo T. | en_US |
dc.contributor.author | Fatma, Nigar | en_US |
dc.date.accessioned | 2006-09-08T20:06:57Z | |
dc.date.available | 2006-09-08T20:06:57Z | |
dc.date.issued | 2003-04 | en_US |
dc.identifier.citation | Kubo, Eri; Singh, Dhirendra P.; Fatma, Nigar; Shinohara, Toshimichi; Zelenka, Peggy; Reddy, Venkat N.; Chylack, Leo T.; (2003). "Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye: loss of LEDGF from nuclei of differentiating cells." Histochemistry and Cell Biology 119(4): 289-299. <http://hdl.handle.net/2027.42/42235> | en_US |
dc.identifier.issn | 0948-6143 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42235 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12692670&dopt=citation | en_US |
dc.description.abstract | Lens epithelium-derived growth factor (LEDGF) enhances the survival and growth of cells. To understand LEDGF's spatial localization and its putative function(s) during proliferation and differentiation, we localized LEDGF during terminal differentiation in whole rat lenses, lens epithelial cell (LEC) explants stimulated with FGF-2, and insulin, iris, human LECs with lentoids. In addition, intracellular localization of LEDGF was performed in other ocular tissues: ciliary body, retina, and cornea. We found the immunopositivity of nuclear LEDGF decreased in LECs of the equatorial region. In contrast, immunopositivity of LEDGF was detected in the cytoplasm of LECs and superficial fiber cells. After treating LEC explants with FGF-2 and insulin, which are known to be differentiating factors for LECs, the nuclei of these cells showed no LEDGF immunopositivity, but explants did express p57 kip2 , a differentiation marker protein. Also, immunopositive LEDGF was not detected in the nuclei of differentiated cells, lentoid body, and corneal epithelial cells. This demonstrated that the loss of LEDGF from the nucleus may be associated with the process of terminal differentiation that might be in some way common with the biochemical mechanisms of apoptosis. The spatial and temporal distribution of LEDGF in the present study also provides a vision for further investigation as to how this protein is involved in cell fate determination. | en_US |
dc.format.extent | 521330 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | LEDGF Immunohistochemistry Differentiation in Situ Hybridization Lentoid Body LEC Explants | en_US |
dc.title | Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye: loss of LEDGF from nuclei of differentiating cells | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Kellogg Eye Center, Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, Michigan, USA, | en_US |
dc.contributor.affiliationother | The Center for Ophthalmic Research, Brigham and Women's Hospital, Havard Medical School, 221 Longwood Avenue, MA 02115, Boston, USA, | en_US |
dc.contributor.affiliationother | Department of Ophthalmology, Room 2009, University of Nebraska Medical Center, NE 68198-6395, Omaha, USA, | en_US |
dc.contributor.affiliationother | Department of Ophthalmology, Room 2009, University of Nebraska Medical Center, NE 68198-6395, Omaha, USA, | en_US |
dc.contributor.affiliationother | Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, MD 20892, Bethesda, USA, | en_US |
dc.contributor.affiliationother | Department of Ophthalmology, Room 2009, University of Nebraska Medical Center, NE 68198-6395, Omaha, USA, | en_US |
dc.contributor.affiliationother | The Center for Ophthalmic Research, Brigham and Women's Hospital, Havard Medical School, 221 Longwood Avenue, MA 02115, Boston, USA, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 12692670 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42235/1/s00418-003-0518-3.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s00418-003-0518-3 | en_US |
dc.identifier.source | Histochemistry and Cell Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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