Whole-cell and single-channel α 1 β 1 γ 2S GABA A receptor currents elicited by a ”multipuffer” drug application device
dc.contributor.author | Greenfield Jr. , L. J. | en_US |
dc.contributor.author | Macdonald, Robert L. | en_US |
dc.date.accessioned | 2006-09-08T20:07:24Z | |
dc.date.available | 2006-09-08T20:07:24Z | |
dc.date.issued | 1996-09 | en_US |
dc.identifier.citation | Greenfield Jr., L. J.; Macdonald, Robert L.; (1996). "Whole-cell and single-channel α 1 β 1 γ 2S GABA A receptor currents elicited by a ”multipuffer” drug application device." Pflügers Archiv European Journal of Physiology 432(6): 1080-1090. <http://hdl.handle.net/2027.42/42242> | en_US |
dc.identifier.issn | 0031-6768 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42242 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8781204&dopt=citation | en_US |
dc.description.abstract | Pharmacological characterization of ion channels and receptors in cultured neurons or transfected cell lines requires microapplication of multiple drug solutions during electrophysiological recording. An ideal device could apply a large number of solutions to a limited area with rapid arrival and removal of drug solutions. We describe a novel ”multipuffer” rapid application device, based on a modified T-tube with a nozzle made from a glass micropipette tip. Drug solutions are drawn via suction from open reservoirs mounted above the recording chamber through the device into a waste trap. Closure of a solenoid valve between the device and the waste trap causes flow of drug solution though the T-tube nozzle. Any number of drug solutions can be applied with rapid onset (50–100 ms) after a brief fixed delay (100–200 ms). Recombinant α 1 β 1 γ 2S GABA A receptors (GABARs) transfected into L929 fibroblasts were recorded using whole-cell and single-channel configurations. Application of GABA resulted in chloride currents with an EC 50 of 12.2 μM and a Hill slope of 1.27, suggesting more than one binding site for GABA. GABAR currents were enhanced by diazepam and pentobarbital and inhibited by bicuculline and picrotoxin. Single-channel recordings revealed a main conductance state of 26–28 pS. This device is particularly suitable for rapid, spatially controlled drug applications onto neurons or other cells recorded in the whole-cell configuration, but is also appropriate for isolated single-channel or multichannel membrane patch recordings. | en_US |
dc.format.extent | 701158 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag Berlin Heidelberg | en_US |
dc.subject.other | Ion Channel | en_US |
dc.subject.other | Key Words Drug Application | en_US |
dc.subject.other | GABAA Receptor | en_US |
dc.subject.other | Electrophysiology | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | Pharmacological Technique | en_US |
dc.subject.other | Superfusion | en_US |
dc.title | Whole-cell and single-channel α 1 β 1 γ 2S GABA A receptor currents elicited by a ”multipuffer” drug application device | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Kinesiology and Sports | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology and Physiology, University of Michigan Medical Center, Michigan, USA, US | en_US |
dc.contributor.affiliationother | Departments of Neurology, Neuroscience Laboratory Building, 1103 E. Huron, Ann Arbor MI 48904-1687, USA, US | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 8781204 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42242/1/424-432-6-1080_64321080.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s004240050238 | en_US |
dc.identifier.source | Pflügers Archiv European Journal of Physiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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