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Effect of anchorage dependency on growth rate and monoclonal antibody production of hybridoma cells
dc.contributor.authorLee, Gyun Minen_US
dc.contributor.authorHuard, Thomas K.en_US
dc.contributor.authorPalsson, Bernhard Øen_US
dc.date.accessioned2006-09-08T20:09:36Z
dc.date.available2006-09-08T20:09:36Z
dc.date.issued1988-05en_US
dc.identifier.citationLee, Gyun Min; Huard, Thomas K.; Palsson, Bernhard O.; (1988). "Effect of anchorage dependency on growth rate and monoclonal antibody production of hybridoma cells." Biotechnology Letters 10(5): 307-312. <http://hdl.handle.net/2027.42/42274>en_US
dc.identifier.issn0141-5492en_US
dc.identifier.issn1573-6776en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/42274
dc.description.abstractHybridoma cells (S3H5/γ2bA2) are found to grow either in suspension or as attached to the surface of cell culture T flask. Cell growth rates and monoclonal antibody (MAB) production rates of both suspended and attached cells were examined. Although the percentage of viable cells was higher for the attached cells, cells growing in suspension showed almost the same charateristics as cells attached to the flasks with respect to cell growth and MAB production rate. Cell attachment increased with increasing serum concentrations up to 5% and remained essentially constant at cell densities of about 2·10 5 /cm 2 .en_US
dc.format.extent425309 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers; Springer Science+Business Mediaen_US
dc.subject.otherChemistryen_US
dc.subject.otherBiotechnologyen_US
dc.subject.otherOrganic Chemistryen_US
dc.subject.otherBioorganic Chemistryen_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherMicrobiologyen_US
dc.subject.otherAnimal Anatomy / Morphology / Histologyen_US
dc.titleEffect of anchorage dependency on growth rate and monoclonal antibody production of hybridoma cellsen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, 48109, Ann Arbor, MIen_US
dc.contributor.affiliationumDepartment of Internal Medicine, University of Michigan, 48109, Ann Arbor, MIen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, 48109, Ann Arbor, MIen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/42274/1/10529_2005_Article_BF01026156.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF01026156en_US
dc.identifier.sourceBiotechnology Lettersen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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