MyoD and myogenin protein expression in skeletal muscles of senile rats
dc.contributor.author | Kostrominova, Tatiana Y. | en_US |
dc.contributor.author | Borisov, Andrei B. | en_US |
dc.contributor.author | Dedkov, Eduard I. | en_US |
dc.contributor.author | Carlson, Bruce M. | en_US |
dc.date.accessioned | 2006-09-08T20:09:47Z | |
dc.date.available | 2006-09-08T20:09:47Z | |
dc.date.issued | 2003-03 | en_US |
dc.identifier.citation | Dedkov, Eduard I.; Kostrominova, Tatiana Y.; Borisov, Andrei B.; Carlson, Bruce M.; (2003). "MyoD and myogenin protein expression in skeletal muscles of senile rats." Cell and Tissue Research 311(3): 401-416. <http://hdl.handle.net/2027.42/42276> | en_US |
dc.identifier.issn | 0302-766X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42276 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12658448&dopt=citation | en_US |
dc.description.abstract | We analyzed the level of protein expression of two myogenic regulatory factors (MRFs), MyoD and myogenin, in senile skeletal muscles and determined the cellular source of their production in young adult (4 months old), old (24, 26, and 28 months old), and senile (32 months old) male rats. Immunoblotting demonstrated levels of myogenin ~3.2, ~4.0, and ~5.5 times higher in gastrocnemius muscles of 24-, 26-, and 32-month-old animals, respectively, than in those of young adult rats. Anti-MyoD antibody recognized two major areas of immunoreactivity in Western blots: a single MyoD-specific band (~43–45 kDa) and a double (or triple) MyoD-like band (~55–65 kDa). Whereas the level of MyoD-specific protein in the 43- to 45-kDa band remained relatively unchanged during aging compared with that of young adult rats, the total level of MyoD-like immunoreactivity within the 55- to 65-kDa bands was ~3.4, ~4.7, ~9.1, and ~11.7 times higher in muscles of 24-, 26-, 28-, and 32-month-old rats, respectively. The pattern of MRF protein expression in intact senile muscles was similar to that recorded in young adult denervated muscles. Ultrastructural analysis of extensor digitorum longus muscle from senile rats showed that, occasionally, the area of the nerve-muscle junction was partially or completely devoid of axons, and satellite cells with the features of activated cells were found on the surface of living fibers. Immunohistochemistry detected accumulated MyoD and myogenin proteins in the nuclei of both fibers and satellite cells in 32-month-old muscles. We suggest that the up-regulated production of MyoD and myogenin proteins in the nuclei of both fibers and satellite cells could account for the high level of MRF expression in muscles of senile rats. | en_US |
dc.format.extent | 960364 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | Aging Skeletal Muscles Muscle Fibers Satellite Cells MyoD Myogenin Rat (WI/HicksCar) | en_US |
dc.title | MyoD and myogenin protein expression in skeletal muscles of senile rats | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Cell and Developmental Biology, 4643 Medical Sciences II Building, University of Michigan, 48109, Ann Arbor, Michigan, USA, | en_US |
dc.contributor.affiliationum | Department of Cell and Developmental Biology, 4643 Medical Sciences II Building, University of Michigan, 48109, Ann Arbor, Michigan, USA, | en_US |
dc.contributor.affiliationum | Department of Cell and Developmental Biology, 4643 Medical Sciences II Building, University of Michigan, 48109, Ann Arbor, Michigan, USA, | en_US |
dc.contributor.affiliationum | Department of Cell and Developmental Biology, 4643 Medical Sciences II Building, University of Michigan, 48109, Ann Arbor, Michigan, USA, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 12658448 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42276/1/s00441-002-0686-9.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s00441-002-0686-9 | en_US |
dc.identifier.source | Cell and Tissue Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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