View Item 

Show simple item record

Effect of mechanical agitation on hybridoma cell growth
dc.contributor.authorLee, Gyun Minen_US
dc.contributor.authorHuard, Thomas K.en_US
dc.contributor.authorKaminski, Mark S.en_US
dc.contributor.authorPalsson, Bernhard Øen_US
dc.date.accessioned2006-09-08T20:10:07Z
dc.date.available2006-09-08T20:10:07Z
dc.date.issued1988-09en_US
dc.identifier.citationLee, Gyun Min; Huard, Thomas K.; Kaminski, Mark S.; Palsson, Bernhard O.; (1988). "Effect of mechanical agitation on hybridoma cell growth." Biotechnology Letters 10(9): 625-628. <http://hdl.handle.net/2027.42/42280>en_US
dc.identifier.issn0141-5492en_US
dc.identifier.issn1573-6776en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/42280
dc.description.abstractHybridoma cells (S3H5/γ2bA2) were grown in spinner flasks at different agitation speeds. It was found that cells in stationary and decline phases of growth were sensitive to shear force caused by agitation but cells in growth phase seemed less sensitive to the shear forces introduced. The death rate was found to be. 0.007 hr −1 in T flasks but 0.018 hr −1 and 0.028 hr −1 at 100 and 200 rpm, respectively, while the growth rate was about 0.05 hr −1 for all cases.en_US
dc.format.extent285763 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers; Springer Science+Business Mediaen_US
dc.subject.otherChemistryen_US
dc.subject.otherBiotechnologyen_US
dc.subject.otherOrganic Chemistryen_US
dc.subject.otherBioorganic Chemistryen_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherMicrobiologyen_US
dc.subject.otherAnimal Anatomy / Morphology / Histologyen_US
dc.titleEffect of mechanical agitation on hybridoma cell growthen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumDepartment of Internal Medicine, University of Michigan, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumDepartment of Internal Medicine, University of Michigan, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/42280/1/10529_2005_Article_BF01024713.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF01024713en_US
dc.identifier.sourceBiotechnology Lettersen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
10529_2005_Article_BF01024713.pdf
Size:
279.0KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.