Effect of calcium chloride treatment on hybridoma cell viability and growth
dc.contributor.author | Lee, Gyun Min | en_US |
dc.contributor.author | Han, Bong Kwan | en_US |
dc.contributor.author | Kim, Jung Hoe | en_US |
dc.contributor.author | Palsson, Bernhard Ø | en_US |
dc.date.accessioned | 2006-09-08T20:12:01Z | |
dc.date.available | 2006-09-08T20:12:01Z | |
dc.date.issued | 1992-10 | en_US |
dc.identifier.citation | Lee, Gyun Min; Han, Bong Kwan; Kim, Jung Hoe; Palsson, Bernhard O.; (1992). "Effect of calcium chloride treatment on hybridoma cell viability and growth." Biotechnology Letters 14(10): 891-896. <http://hdl.handle.net/2027.42/42308> | en_US |
dc.identifier.issn | 0141-5492 | en_US |
dc.identifier.issn | 1573-6776 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42308 | |
dc.description.abstract | In order to minimize hybridoma cell damage during calcium alginate entrapment, the effect of calcium chloride treatment on hybridoma cell viability and growth was studied in terms of calcium chloride concentration and treatment time. The cell viability as measured by trypan blue exclusion did not decrease rapidly during the first hour of calcium chloride treatment regardless of calcium chloride concentrations used (1.3 and 1.5%). However, 1.3% calcium chloride solution appeared to be more detrimental to the cells than 1.5% calcium chloride solution. The cells in 1.3% calcium chloride solution lost their viability faster than the cells in 1.5% calcium chloride solution. In addition, when the cells treated with calcium chloride were inoculated into spinner flasks containing IMDM with 10% fetal calf serum, the cells treated with 1.3% calcium chloride solution showed a longer lag phase than the cells treated with 1.5% calcium chloride solution. | en_US |
dc.format.extent | 405876 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biotechnology | en_US |
dc.subject.other | Organic Chemistry | en_US |
dc.subject.other | Bioorganic Chemistry | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Microbiology | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.title | Effect of calcium chloride treatment on hybridoma cell viability and growth | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, Herbert H. Dow Building, 48109, Ann Arbot, MI | en_US |
dc.contributor.affiliationother | Department of Biotechnology, Korea Advanced Institute of Science, & Technology, Kuseong-Dong, Yuseong-Gu, 305-701, Taejun, Korea | en_US |
dc.contributor.affiliationother | Department of Biotechnology, Korea Advanced Institute of Science, & Technology, Kuseong-Dong, Yuseong-Gu, 305-701, Taejun, Korea | en_US |
dc.contributor.affiliationother | Department of Biotechnology, Korea Advanced Institute of Science, & Technology, Kuseong-Dong, Yuseong-Gu, 305-701, Taejun, Korea | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42308/1/10529_2005_Article_BF01020624.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01020624 | en_US |
dc.identifier.source | Biotechnology Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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