Characterization and Identification of a Human Dentin Phosphophoryn
dc.contributor.author | Clarkson, B. H. | en_US |
dc.contributor.author | Chiego Jr. , D. | en_US |
dc.contributor.author | Chang, S. R. | en_US |
dc.date.accessioned | 2006-09-08T20:15:15Z | |
dc.date.available | 2006-09-08T20:15:15Z | |
dc.date.issued | 1996-09 | en_US |
dc.identifier.citation | Chang, S. R.; Chiego Jr., D.; Clarkson, B. H.; (1996). "Characterization and Identification of a Human Dentin Phosphophoryn." Calcified Tissue International 59(3): 149-153. <http://hdl.handle.net/2027.42/42358> | en_US |
dc.identifier.issn | 0171-967X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42358 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8694890&dopt=citation | en_US |
dc.description.abstract | The present study further characterizes an extract from immature, human tooth apicies from which an intact dentin phosphoprotein has been identified. Third molar apicies from developing roots were decalcified in 10% EDTA until Ca 2+ was undetectable in the decalcifying solution. The crude extract was run on 7.5% SDS-PAGE and stained with ``Stains-All.'' Four distinct bands were found and the molecular weights were 140, 60, 50, and 34 k. When run on a SDS-PAGE under nonreducing conditions the 60, 50, and 34 k bands were absent. These results suggest that the lower molecular weight bands may be subunits of the larger protein. The extract was then further purified by adding CaCl 2 and MgCl 2 to precipitate the phosphoprotein. The precipitate was subjected to a DEAE-Sepharose CL6B column and eluted by 0–0.7 M NaCl gradient solution. The amino acid composition of the purified phosphoprotein was determined and the extract was found to be rich in serine and aspartic acid residues. The N-terminal peptide Asp-Asp-Pro was identified. The sequence of the three amino acids is identical to rat incisor phosphoprotein. | en_US |
dc.format.extent | 166396 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag New York Inc. | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | Key Words: Human — Dentin — Phosphoprotein. | en_US |
dc.title | Characterization and Identification of a Human Dentin Phosphophoryn | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Cariology, Restorative Science, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109, U.S.A., US, | en_US |
dc.contributor.affiliationum | Department of Cariology, Restorative Science, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109, U.S.A., US, | en_US |
dc.contributor.affiliationum | Department of Cariology, Restorative Science, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109, U.S.A., US, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 8694890 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42358/1/223-59-3-149_59n3p149.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s002239900101 | en_US |
dc.identifier.source | Calcified Tissue International | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.