A Novel Method to Isolate Odontoblasts from Rat Incisor
dc.contributor.author | Somerman, Martha J. | en_US |
dc.contributor.author | Davidson, R. M. | en_US |
dc.contributor.author | Guo, L. | en_US |
dc.contributor.author | Berry, J. E. | en_US |
dc.date.accessioned | 2006-09-08T20:15:23Z | |
dc.date.available | 2006-09-08T20:15:23Z | |
dc.date.issued | 2000-03 | en_US |
dc.identifier.citation | Guo, L.; Berry, J. E.; Somerman, M. J.; Davidson, R. M.; (2000). "A Novel Method to Isolate Odontoblasts from Rat Incisor." Calcified Tissue International 66(3): 212-216. <http://hdl.handle.net/2027.42/42360> | en_US |
dc.identifier.issn | 0171-967X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42360 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10666497&dopt=citation | en_US |
dc.description.abstract | Historically, odontoblasts have been isolated from rat incisor using a surgical curette to separate these cells from the dentin. Isolation of odontoblasts using this approach typically resulted in cells with membrane properties that made the application of patch-clamp electrophysiological techniques prohibitive. The studies here describe a new procedure for isolating mature odontoblasts from adult rat incisor to obtain enriched populations of intact, viable odontoblasts that can be readily studied using patch-clamp methodologies. Identification of isolated cells as odontoblasts was confirmed using in situ mRNA hybridization for expression of dentin sialoprotein, osteocalcin, bone sialoprotein, and type I collagen, and calcium flux was monitored in these cells by means of fura-2 microfluorometry. We suggest that either single odontoblasts or clusters of these cells isolated by this new method would be an ideal preparation for the study of odontoblast properties using electrophysiological techniques, in situ hybridization and/or microfluorometry. | en_US |
dc.format.extent | 136212 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag New York Inc. | en_US |
dc.subject.other | Legacy | en_US |
dc.subject.other | Key Words: Odontoblast — Dentin Sialoprotein — Bone Sialoprotein — Osteocalcin — Fura-2. | en_US |
dc.title | A Novel Method to Isolate Odontoblasts from Rat Incisor | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Periodontics/Prevention/Geriatrics, University of Michigan, Ann Arbor, Michigan, USA, US, | en_US |
dc.contributor.affiliationum | Department of Periodontics/Prevention/Geriatrics, University of Michigan, Ann Arbor, Michigan, USA, US, | en_US |
dc.contributor.affiliationother | Division of Basic Sciences, New York University College of Dentistry, 345 East 24th Street, New York, New York 10010, USA, US, | en_US |
dc.contributor.affiliationother | Division of Basic Sciences, New York University College of Dentistry, 345 East 24th Street, New York, New York 10010, USA, US, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 10666497 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42360/1/223-66-3-212_00660212.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/PL00005837 | en_US |
dc.identifier.source | Calcified Tissue International | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.