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Improved Electrochemical Analysis of Neuropathy Target Esterase Activity by a Tyrosinase Carbon Paste Electrode Modified by 1-Methoxyphenazine Methosulfate
dc.contributor.authorSokolovskaya, L. G.en_US
dc.contributor.authorSigolaeva, L. V.en_US
dc.contributor.authorEremenko, A. V.en_US
dc.contributor.authorGachok, I. V.en_US
dc.contributor.authorMakhaeva, G. F.en_US
dc.contributor.authorStrakhova, N. N.en_US
dc.contributor.authorMalygin, V. V.en_US
dc.contributor.authorRichardson, Rudy J.en_US
dc.contributor.authorKurochkin, I. N.en_US
dc.date.accessioned2006-09-08T20:15:55Z
dc.date.available2006-09-08T20:15:55Z
dc.date.issued2005-08en_US
dc.identifier.citationSokolovskaya, L.G.; Sigolaeva, L.V.; Eremenko, A.V.; Gachok, I.V.; Makhaeva, G.F.; Strakhova, N.N.; Malygin, V.V.; Richardson, R.J.; Kurochkin, I.N.; (2005). "Improved Electrochemical Analysis of Neuropathy Target Esterase Activity by a Tyrosinase Carbon Paste Electrode Modified by 1-Methoxyphenazine Methosulfate." Biotechnology Letters 27(16): 1211-1218. <http://hdl.handle.net/2027.42/42368>en_US
dc.identifier.issn0141-5492en_US
dc.identifier.issn1573-6776en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/42368
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16158266&dopt=citationen_US
dc.description.abstractA graphite-paste tyrosinase biosensor was improved by adding 1-methoxyphenazine methosulfate as a mediator. Mediator modification enhanced sensitivity to phenol 4-fold and long-term stability 3-fold. Phenol could be detected at 25 n M (S/N=2) using an Ag/AgCl reference electrode. The biosensor was used to measure the activity of a toxicologically significant enzyme, neuropathy target esterase (NTE), which yields phenol by hydrolysis of the substrate, phenyl valerate. Using the new biosensor, blood and brain NTE inhibition by organophosphorus (OP) compounds with different neuropathic potencies were well correlated ( r =0.990, n =7), supporting the use of blood NTE as a biochemical marker of exposure to neuropathic OP compounds.en_US
dc.format.extent244743 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers; Springeren_US
dc.subject.otherChemistryen_US
dc.subject.otherBiotechnologyen_US
dc.subject.otherApplied Microbiologyen_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherMicrobiologyen_US
dc.subject.otherBioorganic Chemistryen_US
dc.subject.otherBiosensoren_US
dc.subject.otherMediatoren_US
dc.subject.other1-methoxyphenazine Methosulfateen_US
dc.subject.otherNeuropathy Target Esteraseen_US
dc.subject.otherTyrosinaseen_US
dc.titleImproved Electrochemical Analysis of Neuropathy Target Esterase Activity by a Tyrosinase Carbon Paste Electrode Modified by 1-Methoxyphenazine Methosulfateen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumToxicology Program, University of Michigan, 48109, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationotherFaculty of Chemistry, Moscow State University, 119899, Moscow, Russiaen_US
dc.contributor.affiliationotherFaculty of Chemistry, Moscow State University, 119899, Moscow, Russiaen_US
dc.contributor.affiliationotherFaculty of Chemistry, Moscow State University, 119899, Moscow, Russiaen_US
dc.contributor.affiliationotherFaculty of Chemistry, Moscow State University, 119899, Moscow, Russiaen_US
dc.contributor.affiliationotherInstitute of Physiologically Active Compounds, Russian Academy of Sciences, 142432, Moscow Region, Chernogolovka, Russiaen_US
dc.contributor.affiliationotherInstitute of Physiologically Active Compounds, Russian Academy of Sciences, 142432, Moscow Region, Chernogolovka, Russiaen_US
dc.contributor.affiliationotherInstitute of Physiologically Active Compounds, Russian Academy of Sciences, 142432, Moscow Region, Chernogolovka, Russiaen_US
dc.contributor.affiliationotherFaculty of Chemistry, Moscow State University, 119899, Moscow, Russiaen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid16158266en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/42368/1/10529_2005_Article_0020.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/s10529-005-0020-1en_US
dc.identifier.sourceBiotechnology Lettersen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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