Measurement of ammonia and glutamine in cell culture media by gas sensing electrodes
dc.contributor.author | Ozturk, Sadettin S. | en_US |
dc.contributor.author | Meyerhoff, Mark E. | en_US |
dc.contributor.author | Palsson, Bernhard Ø | en_US |
dc.date.accessioned | 2006-09-08T20:23:44Z | |
dc.date.available | 2006-09-08T20:23:44Z | |
dc.date.issued | 1989-04 | en_US |
dc.identifier.citation | Ozturk, Sadettin S.; Meyerhoff, Mark E.; Palsson, Bernhard O.; (1989). "Measurement of ammonia and glutamine in cell culture media by gas sensing electrodes." Biotechnology Techniques 3(4): 217-222. <http://hdl.handle.net/2027.42/42489> | en_US |
dc.identifier.issn | 0951-208X | en_US |
dc.identifier.issn | 1573-6784 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42489 | |
dc.description.abstract | This paper describes the use of a commercially available off-line gas sensing electrode for determination of ammonia and glutamine in cell culture media. The measurement technique was tested in different media preparations with different serum concentrations. The glutamine decomposition was studied as a function of pH for cell culture medium and the results were compared to those obtained by conventional methods, i.e. , HPLC. Finally, glutamine and ammonia metabolism were studied during the cultivation of a hybridoma cell line. | en_US |
dc.format.extent | 335065 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Science & Technology Letters ; Springer Science+Business Media | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Medical Microbiology | en_US |
dc.subject.other | Biotechnology | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.title | Measurement of ammonia and glutamine in cell culture media by gas sensing electrodes | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, The University of Michigan, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, The University of Michigan, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, The University of Michigan, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42489/1/10542_2005_Article_BF01876051.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01876051 | en_US |
dc.identifier.source | Biotechnology Techniques | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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