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Simplified method of making alginate-polylysine microcapsules for hybridoma cell culture using RPMI 1640 medium
dc.contributor.authorLee, Gyun Minen_US
dc.contributor.authorPalsson, Bernhard Øen_US
dc.date.accessioned2006-09-08T20:23:48Z
dc.date.available2006-09-08T20:23:48Z
dc.date.issued1990-09en_US
dc.identifier.citationLee, Gyun Min; Palsson, Bernhard O.; (1990). "Simplified method of making alginate-polylysine microcapsules for hybridoma cell culture using RPMI 1640 medium." Biotechnology Techniques 4(5): 341-344. <http://hdl.handle.net/2027.42/42490>en_US
dc.identifier.issn0951-208Xen_US
dc.identifier.issn1573-6784en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/42490
dc.description.abstractThe method of making alginate-poly-L-lysine microcapsules for hybridoma cell culture can be simplified by cultivating the cells in RPMI 1640 medium. Phosphate concentration in RPMI 1640 medium is sufficiently high to dissolve the alginate gel and, thereby, can be used to eliminate the step of citrate buffer treatment required for reliquefying the interior alginate gel.en_US
dc.format.extent781147 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers; Science & Technology Letters ; Springer Science+Business Mediaen_US
dc.subject.otherChemistryen_US
dc.subject.otherMedical Microbiologyen_US
dc.subject.otherBiotechnologyen_US
dc.subject.otherAnimal Anatomy / Morphology / Histologyen_US
dc.titleSimplified method of making alginate-polylysine microcapsules for hybridoma cell culture using RPMI 1640 mediumen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, 48109, Ann Arbor, MI, USAen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/42490/1/10542_2004_Article_BF00157433.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF00157433en_US
dc.identifier.sourceBiotechnology Techniquesen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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