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Effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridomas during batch culture

dc.contributor.authorLee, Gyun Minen_US
dc.contributor.authorChuck, Alice S.en_US
dc.contributor.authorPalsson, Bernhard Øen_US
dc.date.accessioned2006-09-08T20:23:52Z
dc.date.available2006-09-08T20:23:52Z
dc.date.issued1993-04en_US
dc.identifier.citationLee, Gyun Min; Chuck, Alice S.; Palsson, Bernhard O.; (1993). "Effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridomas during batch culture." Biotechnology Techniques 7(4): 271-276. <http://hdl.handle.net/2027.42/42491>en_US
dc.identifier.issn0951-208Xen_US
dc.identifier.issn1573-6784en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/42491
dc.description.abstractIn order to investigate the effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridoma cells, cells in different growth stages during a batch culture were fixed and stored at 4 and -20 °C, respectively. Flow cytometric analysis indicates that both fixation temperatures can be used in monitoring the changes in intracellular antibody content of the cells during a batch culture. However, it is better to fix and store the cells at -20 °C than 4 °C with regard to preservation of intracellular antibody and storage stability.en_US
dc.format.extent460967 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers; Science & Technology Letters ; Springer Science+Business Mediaen_US
dc.subject.otherChemistryen_US
dc.subject.otherMedical Microbiologyen_US
dc.subject.otherBiotechnologyen_US
dc.subject.otherAnimal Anatomy / Morphology / Histologyen_US
dc.titleEffect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridomas during batch cultureen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, Herbert H. Dow Building, 48109, Ann Arbor, MI, USA; Department of Biotechnology, Korea Advanced Institute of Science & Technology, Kuseong-Dong, 305-701, Yuseong-Gu, Taejun, Koreaen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, Herbert H. Dow Building, 48109, Ann Arbor, MI, USA; Department of Biotechnology, Korea Advanced Institute of Science & Technology, Kuseong-Dong, 305-701, Yuseong-Gu, Taejun, Koreaen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, Herbert H. Dow Building, 48109, Ann Arbor, MI, USA; Department of Biotechnology, Korea Advanced Institute of Science & Technology, Kuseong-Dong, 305-701, Yuseong-Gu, Taejun, Koreaen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/42491/1/10542_2004_Article_BF00150897.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF00150897en_US
dc.identifier.sourceBiotechnology Techniquesen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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