2-Methoxyethanol inhibits gap junctional communication in rat myometrial myocytes
dc.contributor.author | Marty, M. S. | en_US |
dc.contributor.author | Loch-Caruso, Rita | en_US |
dc.date.accessioned | 2006-09-08T20:27:31Z | |
dc.date.available | 2006-09-08T20:27:31Z | |
dc.date.issued | 1998-06 | en_US |
dc.identifier.citation | Marty, M.S.; Loch-Caruso, R.; (1998). "2-Methoxyethanol inhibits gap junctional communication in rat myometrial myocytes." Cell Biology and Toxicology 14(3): 199-210. <http://hdl.handle.net/2027.42/42547> | en_US |
dc.identifier.issn | 0742-2091 | en_US |
dc.identifier.issn | 1573-6822 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42547 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9689493&dopt=citation | en_US |
dc.description.abstract | The glycol ethers 2-methoxyethanol (2-ME) and 2-ethoxyethanol (2-EE) prolong gestation in rodents. Because gap junctions in the myometrium likely facilitate parturition, the present study examined inhibition of gap junctional communication by 2-ME and 2-EE in myometrial smooth-muscle cell cultures. To measure gap junctional communication, the fluorescent dye Lucifer yellow was injected into cultured cells and the transfer of the dye to adjacent cells was scored with epifluorescence microscopy. The data are presented as the percentage of cells adjacent to the microinjected cell that exhibited dye following microinjection. A 30 min treatment with 32 or 63 mmol/L 2-ME decreased dye transfer to 71% and 63%, respectively (p≤0.05; control 90%). Similarly, 2-EE inhibited dye transfer, although myometrial cells were less sensitive to 2-EE compared to 2-ME. Dye transfer returned to control levels after 2 h in the continued presence of 2-ME. The primary metabolite of 2-ME, methoxyacetic acid (MAA), had no effect on dye transfer at concentrations equimolar to 2-ME. Because 2-ME and 2-EE inhibited gap junctional communication only at high concentrations and because the inhibition reversed in the continued presence of the compounds, it is suggested that glycol ethers delay parturition by a mechanism independent of a direct action on myometrial gap junctions. | en_US |
dc.format.extent | 395833 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Pharmacology/Toxicology | en_US |
dc.subject.other | Veterinary Medicine | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.subject.other | 2-ethoxyethanol | en_US |
dc.subject.other | Gap Junctions | en_US |
dc.subject.other | Glycol Ether | en_US |
dc.subject.other | 2-methoxyethanol | en_US |
dc.subject.other | Myometrial Cells | en_US |
dc.subject.other | Uterus | en_US |
dc.title | 2-Methoxyethanol inhibits gap junctional communication in rat myometrial myocytes | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Toxicology Program, Department of Environmental and Industrial Health, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Toxicology Program, Department of Environmental and Industrial Health, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 9689493 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42547/1/10565_2004_Article_168261.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1007414610681 | en_US |
dc.identifier.source | Cell Biology and Toxicology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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