Common origins of MDA-MB-435 cells from various sources with those shown to have melonoma properties
dc.contributor.author | Rae, James Michael | en_US |
dc.contributor.author | Ramus, Susan J. | en_US |
dc.contributor.author | Waltham, Mark | en_US |
dc.contributor.author | Armes, Jane E. | en_US |
dc.contributor.author | Campbell, Ian G. | en_US |
dc.contributor.author | Clarke, Robert | en_US |
dc.contributor.author | Barndt, Robert J. | en_US |
dc.contributor.author | Johnson, Michael D. | en_US |
dc.contributor.author | Thompson, Erik W. | en_US |
dc.date.accessioned | 2006-09-08T20:30:25Z | |
dc.date.available | 2006-09-08T20:30:25Z | |
dc.date.issued | 2004-12 | en_US |
dc.identifier.citation | Rae, James M.; Ramus, Susan J.; Waltham, Mark; Armes, Jane E.; Campbell, Ian G.; Clarke, Robert; Barndt, Robert J.; Johnson, Michael D.; Thompson, Erik W.; (2004). "Common origins of MDA-MB-435 cells from various sources with those shown to have melonoma properties." Clinical & Experimental Metastasis 21(6): 543-552. <http://hdl.handle.net/2027.42/42591> | en_US |
dc.identifier.issn | 0262-0898 | en_US |
dc.identifier.issn | 1573-7276 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42591 | |
dc.description.abstract | Recently, the tissue origin of MDA-MB-435 cell line has been the subject of considerable debate. In this study, we set out to determine whether MDA-MB-435-DTP cells shown to express melanoma-specific genes were identical to various other MDA-MB-435 cell stocks worldwide. CGH-microarray, genetic polymorphism genotyping, microsatellite fingerprint analysis and/or chromosomal number confirmed that the MDA-MB-435 cells maintained at the Lombardi Comprehensive Cancer Center (MDA-MB-435-LCC) are almost identical to the MDA-MB-435-DTP cells, and showed a very similar profile to those obtained from the same original source (MD Anderson Cancer Center) but maintained independently (MDA-MB-435-PMCC). Gene expression profile analysis confirmed common expression of genes among different MDA-MB-435-LCC cell stocks, and identified some unique gene products in MDA-MB-435-PMCC cells. RT-PCR analysis confirmed the expression of the melanoma marker tyrosinase across multiple MDA-MB-435 cell stocks. Collectively, our results show that the MDA-MB-435 cells used widely have identical origins to those that exhibit a melanoma-like gene expression signature, but exhibit a small degree of genotypic and phenotypic drift. | en_US |
dc.format.extent | 289677 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Cancer Research | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Hematology | en_US |
dc.subject.other | Surgical Oncology | en_US |
dc.subject.other | MDA-MB-435 | en_US |
dc.subject.other | Breast Cancer | en_US |
dc.subject.other | Cell Lines | en_US |
dc.subject.other | Melanoma | en_US |
dc.subject.other | Microsatellite Analysis | en_US |
dc.subject.other | Chromosomal Number | en_US |
dc.subject.other | Tyrosinase | en_US |
dc.title | Common origins of MDA-MB-435 cells from various sources with those shown to have melonoma properties | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, , University of Michigan, , , Ann Arbor, , Michigan, , USA, | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, , University of Michigan, , , Ann Arbor, , Michigan, , USA, | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, , University of Michigan, , , Ann Arbor, , Michigan, , USA, | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, , University of Michigan, , , Ann Arbor, , Michigan, , USA, | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, , University of Michigan, , , Ann Arbor, , Michigan, , USA, | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, , University of Michigan, , , Ann Arbor, , Michigan, , USA, | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, , University of Michigan, , , Ann Arbor, , Michigan, , USA, | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, , University of Michigan, , , Ann Arbor, , Michigan, , USA, | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, , University of Michigan, , , Ann Arbor, , Michigan, , USA, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 15679052 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42591/1/10585_2004_Article_DO00003759.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s10585-004-3759-1 | en_US |
dc.identifier.source | Clinical & Experimental Metastasis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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