Attachment, spreading and growth in vitro of highly malignant and low malignant murine fibrosarcoma cells
dc.contributor.author | Varani, James | en_US |
dc.contributor.author | Grimstad, Ivar Amund | en_US |
dc.contributor.author | Knibbs, Randall N. | en_US |
dc.contributor.author | Hovig, Torstein | en_US |
dc.contributor.author | McCoy, J. Philip Jr. | en_US |
dc.date.accessioned | 2006-09-08T20:30:29Z | |
dc.date.available | 2006-09-08T20:30:29Z | |
dc.date.issued | 1985-01 | en_US |
dc.identifier.citation | Varani, James; Grimstad, Ivar Amund; Knibbs, Randall N.; Hovig, Torstein; McCoy, J. Philip; (1985). "Attachment, spreading and growth in vitro of highly malignant and low malignant murine fibrosarcoma cells." Clinical & Experimental Metastasis 3(1): 45-59. <http://hdl.handle.net/2027.42/42592> | en_US |
dc.identifier.issn | 0262-0898 | en_US |
dc.identifier.issn | 1573-7276 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42592 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4042456&dopt=citation | en_US |
dc.description.abstract | Highly malignant cell lines and low-malignant cell lines isolated from three different methylcholanthrene-induced murine fibrosarcomas were examined for their ability to attach to plastic dishes and collagen-coated dishes under serumfree conditions and in the presence of serum. Most of the cells from the three highly malignant lines attached and spread under all conditions. By 72h, there was a significant increase in the number of cells indicating that at least some of the cells had undergone division (even in the absence of serum). In contrast, fewer of the cells from the three low-malignant lines attached and spread on the plastic or collagen substrates in the absence of serum or in the presence of 0.1 per cent serum. However, when 15 μ g laminin per dish was added along with the lowmalignant cells, they then attached and spread on the plastic and collagen-coated dishes. Previous studies have indicated that the highly malignant lines express cell surface antigens that cross-react with laminin while the low-malignant cell lines do not. We speculate that the differences between the high- and low-malignant cells in the expression of cell surface laminin-like antigens contribute to the dissimilarities in attachment and spreading capacity. These differences may also contribute to the dissimilarity between these cells in malignant potential. | en_US |
dc.format.extent | 2084533 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Taylor & Francis Ltd ; Springer Science+Business Media | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Cancer Research | en_US |
dc.subject.other | Hematology | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Surgical Oncology | en_US |
dc.title | Attachment, spreading and growth in vitro of highly malignant and low malignant murine fibrosarcoma cells | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, The University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Pathology, The University of Michigan Medical School, 48109, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | Institute of Pathology, University of Oslo, Rikshospitalet-The National Hospital, N-0027, Oslo 1, Norway | en_US |
dc.contributor.affiliationother | Institute of Pathology, University of Oslo, Rikshospitalet-The National Hospital, N-0027, Oslo 1, Norway | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 4042456 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42592/1/10585_2005_Article_BF01758953.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01758953 | en_US |
dc.identifier.source | Clinical & Experimental Metastasis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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