Use of recombinant and synthetic peptides as attachment factors for cells on microcarriers
dc.contributor.author | Varani, James | en_US |
dc.contributor.author | Inman, Dennis R. | en_US |
dc.contributor.author | Fligiel, Suzanne E. G. | en_US |
dc.contributor.author | Hillegas, William J. | en_US |
dc.date.accessioned | 2006-09-08T20:31:56Z | |
dc.date.available | 2006-09-08T20:31:56Z | |
dc.date.issued | 1993-01 | en_US |
dc.identifier.citation | Varani, James; Inman, Dennis R.; Fligiel, Suzanne E. G.; Hillegas, William J.; (1993). "Use of recombinant and synthetic peptides as attachment factors for cells on microcarriers." Cytotechnology 13(2): 89-98. <http://hdl.handle.net/2027.42/42614> | en_US |
dc.identifier.issn | 0920-9069 | en_US |
dc.identifier.issn | 1573-0778 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42614 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7764582&dopt=citation | en_US |
dc.description.abstract | Polystyrene culture dishes and polystyrene microcarriers were coated with Pronectin-F and poly- l -lysine (polylysine), either alone or in combination. Pronectin-F is a recombinant peptide containing repeats of the RGD cell-attachment sequence from fibronectin. Polylysine is a polymer of l -lysine. Pronectin-F supported attachment of Madin-Darby Canine Kidney (MDCK) cells at concentrations as low as 0.025 μg/cm 2 of surface area. The cells rapidly spread after attachment. Polylysine at concentrations of 0.05–0.5 μg/cm 2 also supported cell attachment but cells did not rapidly spread after attachment to this substrate. Higher concentrations of polylysine could not be used because of toxicity. When the two peptides were used in conjunction, MDCK cells attached and spread at lower peptide concentrations than they did when either substrate was used alone. These findings suggest that recombinant Pronectin-F alone or in conjunction with a cationic polymer could be a useful replacement for materials such as gelatin or collagen which are currently used as microcarrier surfaces. | en_US |
dc.format.extent | 1044975 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Biomedicine General | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Proteomics | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Collagen | en_US |
dc.subject.other | Gelatin | en_US |
dc.subject.other | MDCK | en_US |
dc.subject.other | Microcarrier | en_US |
dc.subject.other | Polylysine | en_US |
dc.subject.other | Pronectin-F | en_US |
dc.title | Use of recombinant and synthetic peptides as attachment factors for cells on microcarriers | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The Department of Pathology, The University of Michigan, 1301 Catherine Rd, Box 0602, 48109, Ann Arbor, Michigan, USA; The Department of Pathology, Wayne State University-VAMC, Allen Park, Michigan, USA; SoloHill Labs, Inc., Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | The Department of Pathology, The University of Michigan, 1301 Catherine Rd, Box 0602, 48109, Ann Arbor, Michigan, USA; The Department of Pathology, Wayne State University-VAMC, Allen Park, Michigan, USA; SoloHill Labs, Inc., Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | The Department of Pathology, The University of Michigan, 1301 Catherine Rd, Box 0602, 48109, Ann Arbor, Michigan, USA; The Department of Pathology, Wayne State University-VAMC, Allen Park, Michigan, USA; SoloHill Labs, Inc., Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | The Department of Pathology, The University of Michigan, 1301 Catherine Rd, Box 0602, 48109, Ann Arbor, Michigan, USA; The Department of Pathology, Wayne State University-VAMC, Allen Park, Michigan, USA; SoloHill Labs, Inc., Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 7764582 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42614/1/10616_2004_Article_BF00749935.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00749935 | en_US |
dc.identifier.source | Cytotechnology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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