Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions
dc.contributor.author | Varani, James | en_US |
dc.contributor.author | Piel, Felicia | en_US |
dc.contributor.author | Josephs, Sean | en_US |
dc.contributor.author | Beals, Theodore F. | en_US |
dc.contributor.author | Hillegas, William J. | en_US |
dc.date.accessioned | 2006-09-08T20:32:20Z | |
dc.date.available | 2006-09-08T20:32:20Z | |
dc.date.issued | 1998-09 | en_US |
dc.identifier.citation | Varani, James; Piel, Felicia; Josephs, Sean; Beals, Ted F.; Hillegas, William J.; (1998). "Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions." Cytotechnology 28 (1-3): 101-109. <http://hdl.handle.net/2027.42/42620> | en_US |
dc.identifier.issn | 0920-9069 | en_US |
dc.identifier.issn | 1573-0778 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42620 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19003412&dopt=citation | |
dc.description.abstract | The present study describes a novel microcarrier substrate consisting of a swellable, copolymer of styrene and divinylbenzene, derivatized with trimethylamine. The co-polymer trimethylamine microcarriers support the growth of a number of different cell lines – Madin Darby Bovine Kidney, Madin-Darby Canine Kidney, Vero and Cos-7 – under serum-free conditions, and human diploid fibroblasts in serum-containing medium. Cells attach to the co-polymer trimethylamine microcarriers as rapidly as they attach to other charged-surface microcarriers (faster than they attach to collagen-coated polystyrene microcarriers) and spread rapidly after attachment. All of the cells examined grow to high density on the co-polymer trimethylamine microcarriers. Furthermore, cells are readily released from the surface after exposure to a solution of trypsin/EDTA. In this respect, the co-polymer trimethylamine microcarriers are different from other charged-surface microcarriers. Madin-Darby Bovine Kidney cells grown on this substrate support production of vaccine strain infectious bovine rhinotracheitis virus as readily as on other charged-surface or collagen-coated microcarriers. Thus, the co-polymer trimethylamine microcarriers combine the positive characteristics of the currently available charged-surface and adhesion-peptide coated microcarriers in a single product. The viral vaccine production industry is undergoing considerable change as manufacturers move toward complete, animal product-free culture systems. This novel substrate should find application in the industry, especially in processes which depend on viable cell recovery. | en_US |
dc.format.extent | 111791 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Biomedicine General | en_US |
dc.subject.other | Biotechnology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Proteomics | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Cell Lines | en_US |
dc.subject.other | Diploid Fibroblasts | en_US |
dc.subject.other | Microcarriers | en_US |
dc.subject.other | Serum-free Culture | en_US |
dc.title | Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, The University of Michigan, 1301 Catherine Road, P.O. Box 0602, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationother | SoloHill Engineering, Inc., Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | SoloHill Engineering, Inc., Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | SoloHill Engineering, Inc., Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | SoloHill Engineering, Inc., Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 19003412 | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42620/1/10616_2004_Article_194743.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1008029715765 | en_US |
dc.identifier.source | Cytotechnology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.