The histochemistry of thiols and disulphides. IV. Protective fixation by organomercurial-formalin mixtures
dc.contributor.author | Sippel, Theodore O. | en_US |
dc.date.accessioned | 2006-09-08T20:47:15Z | |
dc.date.available | 2006-09-08T20:47:15Z | |
dc.date.issued | 1980-01 | en_US |
dc.identifier.citation | Sippel, T. O.; (1980). "The histochemistry of thiols and disulphides. IV. Protective fixation by organomercurial-formalin mixtures." The Histochemical Journal 12(1): 107-117. <http://hdl.handle.net/2027.42/42846> | en_US |
dc.identifier.issn | 0018-2214 | en_US |
dc.identifier.issn | 1573-6865 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42846 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7372500&dopt=citation | en_US |
dc.description.abstract | Formation of mercaptides as the result of adding organomercuric salts to neutral formalin used for fixation was found to protect protein thiols from autoxidation, provided the tissues were washed in distilled and not tap water. Such bloking, in contrast to that given by HgCl 2 , could be reversed quantitatively by mercaptoethanol made strongly acid to keep it from reducing disulphides. However, some cleavage of disulphides by the mercurials themselves caused slight arbfactual thiol staining in a limited number of sites. Three of the nine compounds tested are sufficiently soluble to penetrate tissues with reasonable speed, stable enough to preclude more than incidental mercurial deposits and currently available commercially. Of them, the diuretic mercurial Mersalyl is at present the protecting, agent of choice since methyl- and ethylmercuric chlorides are too toxic to recommend for routine use. | en_US |
dc.format.extent | 1110951 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Chapman and Hall Ltd. ; Springer Science+Business Media | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Biomedicine General | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.subject.other | Biological Microscopy | en_US |
dc.title | The histochemistry of thiols and disulphides. IV. Protective fixation by organomercurial-formalin mixtures | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Anatomy, The University of Michigan, 48109, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 7372500 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42846/1/10735_2005_Article_BF01066541.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01066541 | en_US |
dc.identifier.source | The Histochemical Journal | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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