Microscopical methods for the localization of Na + , K + -ATPase
dc.contributor.author | Ernst, Stephen A. | en_US |
dc.contributor.author | Hootman, Seth R. | en_US |
dc.date.accessioned | 2006-09-08T20:47:32Z | |
dc.date.available | 2006-09-08T20:47:32Z | |
dc.date.issued | 1981-05 | en_US |
dc.identifier.citation | Ernst, Stephen A.; Hootman, Seth R.; (1981). "Microscopical methods for the localization of Na + , K + -ATPase." The Histochemical Journal 13(3): 397-418. <http://hdl.handle.net/2027.42/42850> | en_US |
dc.identifier.issn | 0018-2214 | en_US |
dc.identifier.issn | 1573-6865 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42850 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6265411&dopt=citation | en_US |
dc.description.abstract | Na + , K + -ATPase plays a central role in the ionic and osmotic homeostasis of cells and in the movements of electrolytes and water across epithelial boundaries. Microscopic localization of the enzyme is, therefore, of crucial importance in establishing the subcellular routes of electrolyte flow across structurally complex and functionally polarized epithelia. Recently developed approaches to the localization of Na + , K + -ATPase are reviewed. These methods rely on different properties of the enzyme and encompass cytochemical localization of the K + -dependent nitrophenylphosphatase component of the enzyme, autoradiographic localization of tritiated ouabain binding sites, and immunocytochemical localization of the holoenzyme and of its catalytic subunit. The rationales for each of these techniques are outlined as are the critieria that have been established to validate each method. The observed localization of Na + , K + -ATPase in various tissues is discussed, particularly as it relates to putative and hypothetical mechanisms that are currently thought to mediate reabsorptive and secretory electrolyte transport. | en_US |
dc.format.extent | 1616807 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Chapman and Hall Ltd. ; Springer Science+Business Media | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Biomedicine General | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.subject.other | Biological Microscopy | en_US |
dc.title | Microscopical methods for the localization of Na + , K + -ATPase | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Anatomy, University of Michigan, Medical Sciences II, 48109, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Anatomy, University of Michigan, Medical Sciences II, 48109, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 6265411 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42850/1/10735_2005_Article_BF01005056.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01005056 | en_US |
dc.identifier.source | The Histochemical Journal | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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